Section 3
Chapter 2,216

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

Baril, C.; Herrmann, J.L.; Richaud, C.; Margarita, D.; Girons, I.S.

Journal of Bacteriology 174(23): 7566-7571


ISSN/ISBN: 0021-9193
PMID: 1447129
DOI: 10.1128/jb.174.23.7566-7571.1992
Accession: 002215329

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Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.

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