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Sequence of a cDNA encoding nitrite reductase from the tree Betula pendula and identification of conserved protein regions



Sequence of a cDNA encoding nitrite reductase from the tree Betula pendula and identification of conserved protein regions



M G G: Molecular and general genetics 231(3): 411-416



The sequence of an mRNA encoding nitrite reductase (NiR, EC 1.7.7.1.) from the tree Betula pendula was determined. A cDNA library constructed from leaf poly(A)+ mRNA was screened with an oligonucleotide probe deduced from NiR sequences from spinach and maize. A 2.5 kb cDNA was isolated that hybridized to an mRNA, the steady-state level of which increased markedly upon induction with nitrate. The nucleotide sequence of the cDNA contains a reading frame encoding a protein of 583 amino acids that reveals 79% identity with NiR from spinach. The transit peptide of the NiR precursor from birch was determined to be 22 amino acids in size by sequence comparison with NiR from spinach and maize and is the shortest transit peptide reported so far. A graphical evaluation of identities found in the NiR sequence alignment revealed nine well conserved sections each exceeding ten amino acids in size. Sequence comparisons with related redox proteins identified essential residues involved in cofactor binding. A putative binding site for ferredoxin was found in the N-terminal half of the protein.

Accession: 002219025

Download citation: RISBibTeXText

PMID: 1347145

DOI: 10.1007/bf00292710

Download PDF Full Text: Sequence of a cDNA encoding nitrite reductase from the tree Betula pendula and identification of conserved protein regions



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