+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Sequence of fimbrial subunit-encoding genes from virulent and benign isolates of Dichelobacter (Bacteroides) nodosus

Sequence of fimbrial subunit-encoding genes from virulent and benign isolates of Dichelobacter (Bacteroides) nodosus

Gene 99(1): 115-119

Both virulent and benign isolates of the ovine pathogen Dichelobacter (Bacteroides) nodosus produce polar fimbriae which have been implicated in twitching motility. The fimbrial subunit-encoding genes from two virulent and two benign serogroup-B isolates of D. nodosus were cloned and sequenced. Analysis of the deduced amino acid (aa) sequences of these subunits indicated the presence of substitutions that appeared to correlate with the virulence phenotype. However, these aa substitutions were located in variable regions of the protein where they are unlikely to alter the functional properties of the fimbriae. The aa sequences of the serogroup-B subunits had a very high level (91-95%) of similarity, particularly at the N terminus, where the conserved region extended up to aa 61. Specific aa substitutions within the subunit of one isolate may reflect its serotypic variation from the other serogroup-B subunits studied.

(PDF emailed within 0-6 h: $19.90)

Accession: 002219045

Download citation: RISBibTeXText

PMID: 1673665

DOI: 10.1016/0378-1119(91)90042-A

Related references

Sequence of fimbrial subunit encoding genes from virulent and benign isolates of dichelobacter nodosus equals bacteroides nodosus. Gene (Amsterdam) 99(1): 115-120, 1991

Novel fimbrial subunit genes of Dichelobacter nodosus: recombination in vivo or in vitro?. Veterinary Microbiology 76(2): 163-174, 2000

Molecular cloning of the fimbrial subunit gene from a benign type b isolate of bacteroides nodosus. FEMS Microbiology Letters 33(1): 73-78, 1986

Glycosylation of dichelobacter nodosus fimbrial subunit proteins?. Glycobiology 11(10): 904, October, 2001

A degrading proteinase test to distinguish benign and virulent ovine isolates of Bacteroides nodosus. Australian Veterinary Journal 55(1): 25-28, 1979

Proteinase isoenzyme patterns of Bacteroides nodosus: distinction between ovine virulent isolates, ovine benign isolates and bovine isolates. Journal of General Microbiology 128(4): 809-812, 1982

Transfer of Kingella indologenes (Snell and Lapage 1976) to the genus Suttonella gen. nov. as Suttonella indologenes comb. nov.; transfer of Bacteroides nodosus (Beveridge 1941) to the genus Dichelobacter gen. nov. as Dichelobacter nodosus comb. nov.; and assignment of the genera Cardiobacterium, Dichelobacter, and Suttonella to Cardiobacteriaceae fam. nov. in the gamma division of Proteobacteria on the basis of 16S rRNA sequence comparisons. International Journal of Systematic Bacteriology 40(4): 426-433, 1990

Cloning and expression of Dichelobacter nodosus fimbrial subunit gene in Pseudomonas aeruginosa. Chinese Journal of Veterinary Science 20(4): 362-366, 2000

Dichelobacter nodosus serotype M fimbrial subunit gene: implications for serological classification. Veterinary Microbiology 79(4): 367-374, 2001

Transfer of kingella indologenes snell and lapage 1976 to the genus suttonella new genus as suttonella indologenes new combination transfer of bacteroides nodosus beveridge 1941 to the genus dichelobacter new genus as dichelobacter nodosus new combination and assignment of the genera cardiobacterium dichelobacter and suttonella to cardiobacteriaceae new family in the gamma division of proteobacteria on the basis of 16s ribosomal rna sequence comparisons. International Journal of Systematic Bacteriology 40(4): 426-433, 1990

Detection of the extracellular proteases of Bacteroides nodosus in polyacrylamide gels: a rapid method of distinguishing virulent and benign ovine isolates. Research in Veterinary Science 35(2): 171-174, 1983