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Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae



Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae



Journal of Bacteriology 172(12): 7151-7156



The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in E. coli K-12 and previous MW determinations indicate that this allosteric enzyme is a monomer of MW 51 316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from T. brucei and S. cerevisiae. A potential ATP binding site was conserved in all 3 sequences.

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Accession: 002219080

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PMID: 1701430

DOI: 10.1128/jb.172.12.7151-7156.1990



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