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Sequences 5' to the conserved 28 bp Leg box element regulate the expression of pea seed storage gene legA



Sequences 5' to the conserved 28 bp Leg box element regulate the expression of pea seed storage gene legA



Plant Molecular Biology 15(5): 685-693



Transgenic tobacco plants containing 124 bp of 5' legA (encoding legumin) flanking sequence including the Leg box and all sequences downstream to the start of transcription failed to activate the gene, as judged by ELISA and northern assays. Expression was first detected in a promoter truncation construct which contained 549 bp of 5' flanking sequence. These 2 promoter truncation constructs were additionally examined for their ability to interact with pea seed nuclear proteins.

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