+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Sequencing and characterization of pST1, a cryptic plasmid from Streptococcus thermophilus

Sequencing and characterization of pST1, a cryptic plasmid from Streptococcus thermophilus

FEMS microbiology letters Federation of European Microbiological Societies, 95(2-3): 175-180

Eighty-six strains of S. thermophilus were examined for their plasmid content. Thirteen strains were found to contain one or two plasmids ranging in size from 2.1 to 7.4 kb. DNA-DNA hybridization analysis revealed the presence of five distinct groups of DNA homology. The complete nucleotide sequence of plasmid pST1 (Accession number X65856), which belongs to the major homology group, was determined. It has a molecular size of 2093 bp, a GC content of 35% and contains one major open reading frame of 945 bp (ORF A). The predicted protein, designated Rep A, showed sequence homology with replication proteins from a group of plasmids which are known to replicate via single-stranded DNA intermediates (ssDNA plasmids).

(PDF emailed within 0-6 h: $19.90)

Accession: 002219123

Download citation: RISBibTeXText

PMID: 1526450

DOI: 10.1111/j.1574-6968.1992.tb05362.x

Related references

Identification of the replication region of Streptococcus thermophilus No. 29 plasmid pST1. Bioscience Biotechnology and Biochemistry 57(10): 1646-1649, 1993

Shuttle vectors developed from cryptic plasmid of streptococcus thermophilus. Abstracts of the General Meeting of the American Society for Microbiology 92: 224, 1992

Structural and functional properties of a small cryptic plasmid of Streptococcus thermophilus. Journal of Dairy Science 84(Supplement 1): 307, 2001

Sequence analysis of the replication function of a Streptococcus thermophilus cryptic plasmid pER341. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 291, 1997

Isolation and partial characterization of plasmid DNA from Streptococcus thermophilus. Applied and Environmental Microbiology 50(4): 1103-1106, 1985

Characterization of a theta-replicating plasmid from Streptococcus thermophilus. Plasmid 51(1): 24-36, 2004

Nucleotide sequence of the cryptic plasmid pTT8 from Thermus thermophilus HB8 and isolation and characterization of its high-copy-number mutant. Plasmid 51(3): 227-237, 2004

Yoghurt fermentation at elevated temperatures by strains of Streptococcus thermophilus expressing a small heat-shock protein: application of a two-plasmid system for constructing food-grade strains of Streptococcus thermophilus. Biotechnology Journal 1(4): 398-404, 2006

Characterization of a novel Streptococcus thermophilus rolling-circle plasmid used for vector construction. Applied microbiology and biotechnology 50(2): 174-180, 1998

Sequencing and characterization of the cryptic plasmid QpRS from Coxiella burnetii. Plasmid 44(1): 85-88, 2000

Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid 45(3): 171-183, 2001

Purification, characterization, gene cloning, sequencing, and overexpression of aminopeptidase N from Streptococcus thermophilus A. Applied and Environmental Microbiology 65(7): 3001-3007, 1999