EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Serologic response to the 96 000-Da surface antigen of pathogenic Entamoeba histolytica



Serologic response to the 96 000-Da surface antigen of pathogenic Entamoeba histolytica



Journal of Infectious Diseases 159(4): 794-797



The potential of a purified E. histolytica surface constituent for use as an aid in the diagnosis of amoebiasis, is described. The 96 000 MW antigen (EH-96) was purified by immunoprecipitation from whole amoebic trophozoites using an IgG2b murine MAb followed by excision and electroelution from a 10% SDS-PAGE. Serum samples, tested for reactivity against this antigen using immunoblotting, came from California, and Washington, USA. The 19 patients from California included 15 with invasive disease (10 of whom had E. histolytica cultured from stool or liver abscesses) and 4 asymptomatic homosexual men with non-pathogenic E. histolytica cultured from stool. 32 sera were obtained from Washington, from patients in whom E. histolytica had been considered in the differential diagnosis of patients with symptoms of a liver abscess or intestinal amoebiasis. 20 medical centre workers, also from Washington, with no symptoms, history or risk factors relation to amoebic disease, acted as controls. Ten of 19 sera from Californian patients and 17 of 35 patients from Washington were positive by immunoblot. Of these 54 samples, 44 possessed IHA titres of >=1:256, the diagnostic lower limit for amoebiasis. 50% of all samples and 61% (27 of 44) of these with IHA titres >=256 were positive by immunoblot for EH-96. Of the 54 patients, 33 had accessible medical records. When these patients were stratified by their clinical diagnosis and compared with reactivity to EH-96 by immunoblot, 17 (68%) of 25 patients with invasive disease were positive by immunoblot. The immunoblotting technique had a specificity of 100% when a cut off of >=2 (corresponding to a medium intensity blot) was used. Of the 10 patients from whom E. histolytica was isolated, all 6 with strains that were pathogenic zymodemes were seropositive by immunoblot. The significance of these results for the improved of immunodiagnosis of amoebiasis, is discussed.

(PDF 0-2 workdays service: $29.90)

Accession: 002219349

Download citation: RISBibTeXText

PMID: 2538522



Related references

Structural analysis of the 29 kda surface antigen of pathogenic entamoeba histolytica. Abstracts of the General Meeting of the American Society for Microbiology 92: 122, 1992

An immunogenic 30-kDa surface antigen of pathogenic clinical isolates of Entamoeba histolytica. Journal of Infectious Diseases, 1624: 949-954, 1990

Characterization of an immuno-dominant variable surface antigen from pathogenic and nonpathogenic Entamoeba histolytica. Journal of Experimental Medicine 172(3): 879-888, 1990

cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica. Proceedings of the National Academy of Sciences of the United States of America 87(16): 6358-6362, 1990

Complementary dna sequence analysis of a 29 kda cysteine rich surface antigen of pathogenic entamoeba histolytica. Proceedings of the National Academy of Sciences of the United States of America 87(16): 6358-6362, 1990

Use of monoclonal antibodies to identify, characterize, and purify a 96,000-dalton surface antigen of pathogenic Entamoeba histolytica. Journal of Infectious Diseases 156(2): 334-343, 1987

The serologic response of patients with amebiasis compared by gel-diffusion, hemagglutination, and phagocytosis techniques with a common Entamoeba histolytica antigen preparation. Journal of Laboratory and Clinical Medicine 69(3): 467-471, 1967

Structural analysis and demonstration of the 29 kDa antigen of pathogenic Entamoeba histolytica as the major accessible free thiol-containing surface protein. Molecular Microbiology 7(5): 755-763, 1993

Modulation of a surface antigen of Entamoeba histolytica in response to bacteria. Infection and Immunity 60(4): 1711-1713, 1992

Molecular cloning of a 30-kilodalton lysine-rich surface antigen from a nonpathogenic Entamoeba histolytica strain and its expression in a pathogenic strain. Infection and Immunity 63(3): 917-925, 1995

Stable transformation of a gene encoding a lysine-rich surface antigen from a nonpathogenic Entamoeba histolytica strain into a pathogenic strain. Journal of Eukaryotic Microbiology 44(1): 38A, 1997

The 96-kilodalton antigen as an integral membrane protein in pathogenic Entamoeba histolytica: potential differences in pathogenic and nonpathogenic isolates. Infection and Immunity 58(3): 753-760, 1990