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Serologic response to the 96 000-Da surface antigen of pathogenic Entamoeba histolytica

Serologic response to the 96 000-Da surface antigen of pathogenic Entamoeba histolytica

Journal of Infectious Diseases 159(4): 794-797

The potential of a purified E. histolytica surface constituent for use as an aid in the diagnosis of amoebiasis, is described. The 96 000 MW antigen (EH-96) was purified by immunoprecipitation from whole amoebic trophozoites using an IgG2b murine MAb followed by excision and electroelution from a 10% SDS-PAGE. Serum samples, tested for reactivity against this antigen using immunoblotting, came from California, and Washington, USA. The 19 patients from California included 15 with invasive disease (10 of whom had E. histolytica cultured from stool or liver abscesses) and 4 asymptomatic homosexual men with non-pathogenic E. histolytica cultured from stool. 32 sera were obtained from Washington, from patients in whom E. histolytica had been considered in the differential diagnosis of patients with symptoms of a liver abscess or intestinal amoebiasis. 20 medical centre workers, also from Washington, with no symptoms, history or risk factors relation to amoebic disease, acted as controls. Ten of 19 sera from Californian patients and 17 of 35 patients from Washington were positive by immunoblot. Of these 54 samples, 44 possessed IHA titres of >=1:256, the diagnostic lower limit for amoebiasis. 50% of all samples and 61% (27 of 44) of these with IHA titres >=256 were positive by immunoblot for EH-96. Of the 54 patients, 33 had accessible medical records. When these patients were stratified by their clinical diagnosis and compared with reactivity to EH-96 by immunoblot, 17 (68%) of 25 patients with invasive disease were positive by immunoblot. The immunoblotting technique had a specificity of 100% when a cut off of >=2 (corresponding to a medium intensity blot) was used. Of the 10 patients from whom E. histolytica was isolated, all 6 with strains that were pathogenic zymodemes were seropositive by immunoblot. The significance of these results for the improved of immunodiagnosis of amoebiasis, is discussed.

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Accession: 002219349

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PMID: 2538522

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