EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Serotype O157:H7 Escherichia coli from bovine and meat sources



Serotype O157:H7 Escherichia coli from bovine and meat sources



Journal of Clinical Microbiology 29(6): 1225-1231



Serotype O157:H7 Escherichia coli strains from several different bovine and meat (beef) sources were studied to determine the diversity of their virulence properties and to compare their plasmid characteristics. Eighteen strains from cattle feces, 2 from water buffalo feces, 3 from beef samples, and 2 from feces of human hemolytic uremic syndrome cases were examined. All of these strains hybridized with the CVD419 DNA probe which identifies serotype O157:H7 and many other serotypes of verocytotoxin-producing E. coli. Of 15 bovine strains that hybridized with two verocytotoxin DNA probes, 8 hybridized with both verocytotoxin 1 (VT1) and VT2 probes, 5 hybridized with only the VT2 probe, and 2 hybridized with only the Vt1 probe. This distribution was similar to that reported for O157:H7 E. coli isolated from humans. All three beef isolates hybridized with both VT1 and VT2 probes. All strains that hybridized with the VT probes were positive in the verocytotoxin assay, and all probe-negative strains were negative in the assay. All the strains possessed large plasmids with molecular sizes ranging from 53 to 64 MDa. Fifteen of the 20 cattle and water buffalo strains had one or more additional small plasmids. Restriction patterns resulting from HindIII, SmaI, and BamHI digestions of the large plasmids were used to compare all possible pairs of five different single plasmid-bearing strains from different countries (Egypt, England, and the United States). The restriction patterns of these strains were distinct, and the mean coefficients of similarity for these comparisons ranged from 71 to 91%, indicating a moderate degree of genetic diversity. This diversity and the presence of multiple plasmids in many bovine and human O157:H7 strains reinforce the usefulness of plasmid analysis in future studies. Only four of the 20 bovine strains and 1 of the 3 beef strains possessed the capability for adherence to HEp-2 and Intestine 407 cells in the presence of mannose, indicating that in vitro expression of localized adherence is not a universal property of O157:H7 strains of bovine origin.

(PDF 0-2 workdays service: $29.90)

Accession: 002219629

Download citation: RISBibTeXText

PMID: 1677942



Related references

Prevalence of enterohaemorrhagic Escherichia coli from serotype O157 and other attaching and effacing Escherichia coli on bovine carcasses in Algeria. Journal of Applied Microbiology 101(2): 361-368, 2006

A note on Escherichia coli O157:H7 serotype in Turkish meat products. Meat Science 56(4): 331-335, 2000

Persistence of bovine shedding of Escherichia coli serotype O157:H7 PFGE strains. Proceedings of a meeting of the Society for Veterinary Epidemiology and Preventive Medicine, University of Reading, 29-31 March 1995: 27-32, 1995

An improved screening method for the detection and isolation of escherichia coli o157 h7 from meat incorporating the 3m petrifilm test kit hec for hemorrhagic escherichia coli o157 h7. Journal of Food Protection 53(11): 936-940, 1990

The enterohaemorrhagic Escherichia coli (serotype O157:H7) Tir molecule is not functionally interchangeable for its enteropathogenic E. coli (serotype O127:H6) homologue. Cellular Microbiology 3(8): 499-510, 2001

Stationary-phase acid resistance and injury of recent bovine Escherichia coli O157 and non-O157 biotype I Escherichia coli isolates. Journal of food protection 67(3): 583-590, 2004

Isolation of Escherichia coli O157:H7 from bovine faeces and raw meat products in the Netherlands. Tijdschrift voor Diergeneeskunde 121(11): 324-325, 1996

Detection of Escherichia coli O157 in bovine meat products in northern Italy. International journal of food microbiology1 90(3): 257-262, 2004

Isolation and characterization of Escherichia coli O157 in bovine meat products and cattle in the province of Tucuman. Revista Argentina de Microbiologia 47(2): 125-131, 2015

Isolation of escherichia coli serotype o157 h7 and other shiga like toxin producing escherichia coli from dairy cattle. Journal of Clinical Microbiology 29(5): 985-989, 1991

Temperature range for growth of escherichia coli serotype o157 h7 and selected coliforms in escherichia coli medium. Journal of Clinical Microbiology 28(4): 803-805, 1990