EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Serum resistance of metacyclic stage Leishmania major promastigotes is due to release of C5b-9



Serum resistance of metacyclic stage Leishmania major promastigotes is due to release of C5b-9



Journal of Immunology 145(12): 4311-4316



The mechanism of serum resistance for infective promastigotes of Leishmania major was investigated. Prior results suggested that the mechanism of resistance was mediated at a step after C3 deposition. Equivalent amounts of C3b were deposited on serum-susceptible, noninfective promastigotes harvested from log stage cultures (LOG) and on C-resistant, infective, metacyclic promastigotes (MP) purified from stationary stage cultures. Whereas binding of C9 to LOG was stable during incubation in serum, C9 binding to MP was minimal and unstable, because molecules bound initially to MP were released with continued incubation. Failure to bind C9 was not a result of inability to activate C; the kinetics of C3, C6, and C9 consumption were similar for LOG and MP. Deposition of C5b-7 on MP was stable, indicating that the initial steps in terminal complex formation were intact. Instead the majority of C5b-9 formed on MP was spontaneously released into the serum as SC5b-9. Residual C5b-9 on MP was released with 1 M NaCl. These data show that developmental modification of the promastigote membrane during transition from a noninfective to an infective stage blocks insertion of lytic C5b-9 into the promastigote membrane.

(PDF 0-2 workdays service: $29.90)

Accession: 002219859

Download citation: RISBibTeXText

PMID: 2147941



Related references

The cr1 receptor mediates entry of the metacyclic stage of leishmania major promastigotes in human macrophages. FASEB Journal 2(4): ABSTRACT 3425, 1988

Genes selectively expressed in the infectious (metacyclic) stage of Leishmania major promastigotes encode a potential basic-zipper structural motif. Molecular and Biochemical Parasitology, 522: 241-250, 1992

Intradermal inoculations of low doses of Leishmania major and Leishmania amazonensis metacyclic promastigotes induce different immunoparasitic processes and status of protection in BALB/c mice. International Journal for Parasitology 33(12): 1373-1383, 2003

Leishmania major: identification of developmentally regulated proteins in procyclic and metacyclic promastigotes. Experimental Parasitology 119(3): 422-429, 2008

Development of metacyclic Leishmania promastigotes is associated with the increasing expression of GP65, the major surface antigen. Parasite Immunology (Oxford): 113: 197-209, 1989

CR1, the C3b receptor, mediates binding of infective Leishmania major metacyclic promastigotes to human macrophages. Journal of Immunology 143(2): 617-622, 1989

Behavior of Leishmania major metacyclic promastigotes during the course of infection and immune response development in resistant versus susceptible hosts. Brazilian Journal of Microbiology 34(Supp 1): 17-20, 2003

The generation of infective stage Leishmania major promastigotes is associated with the cell-surface expression and release of a developmentally regulated glycolipid. Journal of Immunology 139(9): 3099-3106, 1987

Leishmania donovani metacyclic promastigotes: transformation in vitro, lectin agglutination, complement resistance, and infectivity. Experimental Parasitology, 642: 147-156, 1987

Leishmania (Leishmania) amazonensis infection and dissemination in mice inoculated with stationary-phase or with purified metacyclic promastigotes. Parasitology 134(Pt 12): 1699-1707, 2007

The gene B protein localises to the surface of Leishmania major parasites in the absence of metacyclic stage lipophosphoglycan. Journal of Cell Science 108: 3359-3366, 1995