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Solution hybridization assay for detecting genetically engineered microorganisms in environmental samples



Solution hybridization assay for detecting genetically engineered microorganisms in environmental samples



Biotechniques 8(3): 316-318



The detection method involves recovery of DNA from the microbial community of an environmental sample followed by hybridization in solution with a radiolabelled RNA gene probe. After nuclease digestion of non-hybridized probe RNA, the DNA-RNA hybrids formed in the solution hybridization reaction are separated by Sephadex or hydroxyapatite column chromatography, and detected by liquid scintillation counting.

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Accession: 002224136

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PMID: 2331372


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