Somatic hybridization in Hordeum species

Funatsuki, H.; Lazzeri, P.A.; Lorz, H.

Physiologia Plantarum 82(1): A22

1991


ISSN/ISBN: 0031-9317
Accession: 002224342

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Abstract
Liquid cultures were established from callus derived from immature embryos of H. marinum, a wild species with stress resistance. The frequency of plant regeneration from these cells was high and morphogenic capacity was retained for >1 year. Suspension cultures were initiated from H. vulgare cell lines, including one transformed with a gene that might function as a selectable marker in somatic hybridization. Protoplast fusion between H. vulgare and H. marinum was carried out using polyethylene glycol. Culture and selection of the fusion products are in progress.