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The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro

, : The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro. Journal of Virology 65(2): 649-656

The E1 open reading frame of bovine papillomavirus type 1 encodes factors necessary for extrachromosomal maintenance of the viral genome in transformed cells. To facilitate biochemical characterization of the gene products encoded by this open reading frame, we have expressed the full-length E1 protein in a baculovirus-insect cell system. This protein was found to be phosphorylated and localized to the nucleus of infected cells. The E1 protein alone has affinity for DNA but appears to lack specificity for viral sequences. In addition, we present evidence that the E1 protein interacts with the virally encoded transcriptional activator E2 in vitro. These results are consistent with a model in which the E1 protein, as part of a complex with E2, interacts with specific DNA sequences in the viral genome.

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Accession: 002241224

PMID: 1846189

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Druback Gregory W.; Arlotta Salvatore V., 1985: Subsurface pollution containment using a composite system vertical cut-off barrier. Pages 400-411 1985

Kouame, Kg; Patureau-Mirand, P; Troccon, Jl; Prugnaud, J; Journet, M; Pion, R., 1984: Calf nutrition during the weaning period. Annales de zootechnie3(4): 445-465

Loof, P. A. A., 1964: Four new species of Criconemoides from the Netherlands. Four new species of Criconemoides from The Netherlands are described and figured. In C. vadensis n.sp. from moist clayey meadow soil, the female is distinguished by the 70 to 81 body annules, the posterior part of the body being conoid, the head n...

Lipski S.W.R.; Do H.L.; Szmigielski S.; Pulverer G.; Jeljaszewicz J., 1981: Post irradiation recovery of hemopoiesis in mice treated with propionibacterium granulosum. The effect of P. granulosum on spontaneous regeneration of hemopoiesis in nonlethally (250 or 400 rad) irradiated mice was investigated and measured by peripheral blood leukocyte counts every 2 days after irradiation and incorporation of 3H-thymid...

Pradhan, S.; Sharma, A.K.; Sopory, S.K., 1993: Cloning of the BamHI repeat from Amaranthus and study of its methylation in genomic DNA during dedifferentiation. A 670 bp BamHI repeat was isolated from the genomic DNA of Amaranthus paniculatus and was cloned in pUC19 vector (pABHs). The level of methylation of total genomic DNA and the BamHI repeat therein was studied by employing methylation sensitive enz...

Grove, D.; Bollwahn, W.; Mahler, R., 1968: Experiments on collection and deep-freeze preservation of semen from the tail of the epididymis. Cannulation of the vas deferens is possible in boars. However, semen collected from the fistula opening by electrodes applied to the scrotal skin in 5 boars was not of sufficiently good quality to justify preservation. 4-10 ml. semen of good quali...

Franke D.; Mey U.; Lachhein L., 1985: Studies on iatrogenic folic acid deficiency anemia in chronic renal insufficiency. The turbidimetric determination of folic acid concentration in serum and erythrocytes was made by means of a spectrophotometer multiscan in 35 conservatively treated patients with chronic renal failure with a minimum creatine retention of 390 .mu....

Vlodavsky I.; Inbar M.; Sachs L., 1972: Temperature sensitive agglutinability of human erythrocytes by lectins. Biochimica et Biophysica Acta 274(2): 364-369

Ferrini, A.M.; Mannoni, V.; Aureli, P.; Salvatore, G.; Piccirilli, E.; Ceddia, T.; Pontieri, E.; Sessa, R.; Oliva, B., 2006: Melaleuca alternifolia essential oil possesses potent anti-staphylococcal activity extended to strains resistant to antibiotics. Melaleuca alternifolia Cheel essential oil (TTO) and its major component terpinen-4-ol were examined against a large number of clinical isolates of Staphylococcus aureus to establish their anti-staphylococcal activities. Classic and established pr...

Gram, Theodore E.; Fouts, James R., 1966: Effect of a -tocopherol upon lipid peroxidation and drug metabolism in hepatic microsomes. In an in vitro system consisting of rat liver 9000g supernatant fraction and a TPNH [reduced triphosphopyridine nucleotide]-generating system, significant lipid peroxidation was observed during incubation at 37[degree]C; negligible peroxidation oc...