EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The effects of dietary ligands on zinc uptake at the porcine intestinal brush-border membrane






British Journal of Nutrition 64(3): 733-741

The effects of dietary ligands on zinc uptake at the porcine intestinal brush-border membrane

Intestinal brush-border-membrane vesicles were prepared from the porcine small bowel by magnesium precipitation and differential centrifugation, and were functionally intact. The influence of dietary ligands on 65Zn uptake was determined using a 65Zn concentration of 5 micromoles, an incubation time of 1 min and a reaction temperature of 27 degrees, with a rapid filtration technique. At this low Zn concentration the addition of an excess of folate, histidine or glucose had no effect on Zn uptake. Addition of picolinate, citrate and phytate to the incubation medium significantly reduced Zn uptake at all concentrations of ligand examined. Any inhibitory effects of folic acid in vivo may thus be due to a mucosal rather than lumen interaction. Those ligands inhibiting absorption may have done so through the formation of Zn-ligand complexes, which are either insoluble, or which reduce the binding of Zn to its mucosal receptor. This in vitro model of Zn absorption is useful for comparing the effects of potential Zn-binding ligands in the diet.


Accession: 002249034

PMID: 2265181

DOI: 10.1079/bjn19900075



Related references

Effects of dietary fat on lipid composition and iron uptake of intestinal brush-border membrane of rats. Journal of nutritional biochemistry 3(3): 135-139, 1992

Zinc uptake into intestinal brush border membrane vesicles of alcoholic pigs. American Journal of Clinical Nutrition 47(4): 783, 1988

Zinc inhibition of sodium stimulated glucose uptake by intestinal brush border membrane vesicles. Federation Proceedings: 1081, 1987

Effect of calcium and other divalent cations on zinc uptake by rat small intestinal brush border membrane. Gastroenterology 108(4 SUPPL ): A728, 1995

Copper uptake and its interaction with zinc and iron in intestinal brush border membrane vesicles in the developing piglet. FASEB Journal 7(3-4): A299, 1993

Dietary phenolic compounds: inhibition of Na+- dependent D-glucose uptake in rat intestinal brush border membrane vesicles. Journal of nutrition 119(11): 1698-1704, 1989

Zinc uptake in swine intestinal brush border membrane vesicles using a 65Zn/69mZn duel isotope experiment. Journal of Radioanalytical and Nuclear Chemistry 276(1): 129-134, 2008

Dietary phenolic compounds inhibition of sodium dependent d glucose uptake in rat intestinal brush border membrane vesicles. Journal of Nutrition 119(11): 1698-1704, 1989

Effects of zinc and copper on cadmium uptake by brush border membrane vesicles. Toxicology Letters (shannon). 91(2): 111-120, 1997

Effects of zinc on L- proline uptake by lobster hepatopancreatic brush-border membrane vesicles. Journal of Experimental Biology 202(21): 3003-3010, 1999