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The epitope of a protective monoclonal antibody occurs in a region of microheterogeneity in Plasmodium chabaudi






Molecular and Biochemical Parasitology 42(1): 153-154

The epitope of a protective monoclonal antibody occurs in a region of microheterogeneity in Plasmodium chabaudi

The nucleotide sequence of the homologous region of the MSA1 (major merozoite surface antigen precursor) genes from P. chabaudi adami DK and P. chabaudi chabaudi CB is reported and compared with that in P. chabaudi adami DS. The alignment of the predicted protein sequence of this segment is given. There is a single nucleotide difference between DS and DK. The amino acid homology between DS and DK, CB, P. yoelii YM and P. falciparum Fc27 is 99.8, 77, 53 and 25% respectively. The single amino acid change between DS and DK (Thr to Ile) resides in the core epitope region of the protective monoclonal antibody 5C10/66. This is compared to a similar region of microheterogeneity between P. falciparum Fc27 and P. falciparum Camp. It is suggested that regions of microheterogeneity are selected for to evade protective immune responses.

Accession: 002249995

PMID: 1700297

DOI: 10.1016/0166-6851(90)90123-4

Download PDF Full Text: The epitope of a protective monoclonal antibody occurs in a region of microheterogeneity in Plasmodium chabaudi



Related references

Lew, A.M.; Beck, D.J., 1990: The epitope of a protective monoclonal antibody occurs in a region of microheterogeneity in Plasmodium chabaudi. Molecular and Biochemical Parasitology 42(1): 153-154

Lew, A.M.; Langford, C.J.; Anders, R.F.; Kemp, D.J.; Saul, A.; Fardoulys, C.; Geysen, M.; Sheppard, M., 1989: A protective monoclonal antibody recognizes a linear epitope in the precursor to the major merozoite antigens of Plasmodium chabaudi adami. The monoclonal antibody 5C10/66 was shown to afford strong protection in mice against fulminating Plasmodium chabaudi adami infection. This was remarkable, as immunity to this organism is regarded to be mainly T-cell mediated. This antibody identi...

Lew, A.M.; Beck, D.J.; Thomas, L.M., 1990: A second region recognized by the protective monoclonal antibody 5C10/66 in the precursor to the major merozoite surface antigen of Plasmodium chabaudi adami. Molecular and Biochemical Parasitology 41(2): 289-291

Lew, A.M.; Beck, D.J.; Thomas, L.M., 1990: A second region recognized by the protective monoclonal antibody 5C10/66 in the precursor to the major merozoite surface antigen of Plasmodium chabaudi adami. The sequence of a lambda gt11 cDNA clone from P. chabaudi adami DS that was selected using monoclonal antibody 5C10/66 is reported. This 0.8 kb fragment (3103) was part of the MSA-1 gene, but did not overlap with a 1.4 kb fragment described previo...

Wood, J.C.; Sales de Aguiar, J.C.; Jarra, W.; Ogun, S.A.; Snounou, G.; Brown, K.N., 1989: In vivo selection of populations of Plasmodium chabaudi chabaudi AS resistant to a monoclonal antibody that reacts with the precursor to the major merozoite surface antigen. Mice bearing a hybridoma secreting a monoclonal antibody (MAb), MAb-3, which significantly delays the onset of a Plasmodium chabaudi chabaudi AS, but not P. chabaudi chabaudi CB, challenge parasitemia in a passive transfer assay and which is speci...

Jones, T.R.; Yuan, L.F.; Marwoto, H.A.; Gordon, D.M.; Wirtz, R.A.; Hoffman, S.L., 1992: Low immunogenicity of a Plasmodium vivax circumsporozoite protein epitope bound by a protective monoclonal antibody. The repeat region of the Plasmodium vivax circumsporozoite (CS) protein contains 20 copies of the nine-amino acid sequence DRA A/D GQPAG. A monoclonal antibody that passively protects monkeys against sporozoite challenge recognizes a four-amino ac...

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Burns, J.M.; Daly, T.M.; Vaidya, A.B.; Long, C.A., 1988: The 3' portion of the gene for a Plasmodium yoelii merozoite surface antigen encodes the epitope recognized by a protective monoclonal antibody. The 230-kDa merozoite antigen of the murine malarial parasite Plasmodium yoelii provides a potential model system for the development of a protective erythrocytic stage vaccine. To characterize this antigen at the molecular level, isolated P. yoel...

Jarra, W.H.lls, L.; March, J.; Brown, K., 1986: Protective immunity to malaria. Studies with cloned lines of Plasmodium chabaudi chabaudi and Plasmodium berghei in CBA. Parasite immunology 8(3): 239-254