The production of fertile triploid hybrids from crosses between Hordeum vulgare L. (2n = 4x = 28) and H. bulbosum L. (2n = 2x = 14)

Pickering, R.A.

Hereditas Landskrona 114(3): 227-236

1991


DOI: 10.1111/j.1601-5223.1991.tb00329.x
Accession: 002256745

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Abstract
Triploid interspecific hybrids (2n = 3x = 21) combining 2 sets of chromosomes from H. vulgare and one from H. bulbosum have twice been reported previously, with differing accounts of chromosome pairing at meiotic metaphase I. In attempts to produce large numbers of these hybrids, reciprocal crosses were made between 2 tetraploid cultivars of barley (H. vulgare 2n = 4x = 28) and 4 diploid genotypes of H. bulbosum (2n = 2x = 14) in an environment known to promote increased retention of the H. bulbosum chromosomes. Forty-five putative hybrids were regenerated after embryo culture from H. vulgare (female) X H. bulbosum (male). Their chromosome numbers ranged from 19 to 22. At metaphase I, 7 bivalents plus varying numbers of univalents were frequently observed. Trivalents were rarely seen and probably arose from autosyndetic pairing of H. vulgare chromosomes. Of 36 hybrids inoculated with 3 fungal pathogens (powdery mildew (Erysiphe graminis), net blotch (Dreschlera teres) and scald (Rhynchosporium secalis)), 13 were resistant, but progeny from hybrids were susceptible. The lack of allosyndetic pairing makes it doubtful whether the hybrids will be of practical value for gene introgression from H. bulbosum to H. vulgare. From the reciprocal cross 3 plants were obtained which were morphologically similar to H. bulbosum but 2 had reduced height. As all 3 showed lower pairing at metaphase I than H. bulbosum, they may have arisen parthenogenetically.

The production of fertile triploid hybrids from crosses between Hordeum vulgare L. (2n = 4x = 28) and H. bulbosum L. (2n = 2x = 14)