+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in the Netherlands



The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in the Netherlands



Veterinary Microbiology 26(1/2): 25-40



Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002260914

Download citation: RISBibTeXText

PMID: 1850889

DOI: 10.1016/0378-1135(91)90039-i


Related references

The use of elisa systems for serology and antigen detection in parvovirus coronavirus and rotavirus infections in dogs in the netherlands. Veterinary Microbiology 26(1-2): 25-40, 1991

Evaluation of enzyme-linked immunosorbent assays based on monoclonal antibodies for the serology and antigen detection in canine parvovirus infections. Veterinary Quarterly 12(1): 14-20, 1990

An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat. Comparative Immunology, Microbiology and Infectious Diseases 12(4): 139-146, 1989

Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs. Veterinary Microbiology 26(1/2): 41-51, 1991

Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay. Clinical and Diagnostic Laboratory Immunology 6(1): 130-132, 1999

Enzyme linked immunosorbent assay for detection of porcine rotavirus antigen in fecal samples. Agri Practice 7(6): 7-8, 10, 1986

Occurrence of rotavirus infection in the dog. Antigen and antibody detection by means of enzyme linked immunosorbent assay. Zum Vorkommen der Rotavirus Infektion beim Hund Antigen und Antikorpernachweis mittels Enzyme Linked Immunosorbent Assay ELISA: 117, 1983

Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 27(2): 245-250, 1989

Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 17(6): 997, 1983

Comparison of enzyme-linked immunosorbent assay, DNA hybridization, hemagglutination, and electron microscopy for detection of canine parvovirus infections. Journal of Clinical Microbiology 20(3): 373-378, 1984

Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies. Journal of Virological Methods 24(1-2): 43-56, 1989

Application of enzyme-linked immunosorbent assay (ELISA) to the detection of calf rotavirus and rotavirus antibodies. Annales de Microbiologie 128a(4): 499-510, 1977

Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus. Clinical and Diagnostic Laboratory Immunology 12(3): 474-476, 2005

Detection of circulating antigen during acute infections with Toxoplasma gondii by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 6(6): 545-547, 1977

Specificity of commercially available Entamoeba histolytica antigen in enzyme-linked immunosorbent assay (ELISA) to sera from dogs with various parasitic infections. Journal of Veterinary Medical Science 53(4): 729-731, 1991