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Tocopherol content of selected foods by HPLC/fluorescence quantitation



Tocopherol content of selected foods by HPLC/fluorescence quantitation



Journal of Food Composition and Analysis 2(3): 200-209



A non-aqueous reverse-phase HPLC system was used to estimate alpha -, gamma - and delta -tocopherol isomers in 40 food products important to the USA diet. Samples were saponified with potassium hydroxide overnight and extracted with hexane. A Zorbax ODS (5 micro m) column and acetonitrile/methylene chloride/methanol (700:300:50) mobile phase were used with fluorescence excitation at 290 nm and emission at 330 nm.

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Accession: 002263533

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DOI: 10.1016/0889-1575(89)90017-3



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