+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcription of the Petunia mitochondrial CMS-associated Pcf locus in male sterile and fertility-restored lines

Transcription of the Petunia mitochondrial CMS-associated Pcf locus in male sterile and fertility-restored lines

M G G: Molecular and general genetics 227(3): 348-355

Transcripts of the Petunia mitochondrial cytoplasmic male sterility (CMS)-associated S-Pcf locus, which consists of three co-transcribed genes (pcf, NADH dehydrogenase subunit 3, and ribosomal protein S12), have been characterized in reproductive tissues of CMS and fertility restored (Rf) Petunia lines by nuclease protection experiments and by RNA blot hybridization. Three 5' transcript termini have been previously described. Two 3' transcript termini and an additional S-Pcf locus transcript have now been identified. The relative abundance of the three 5' transcript termini is influenced by the presence of the nuclear Rf gene. A decrease in the abundance of the -121 5' transcript terminus relative to the -266 and -522 termini is consistently seen in Petunia lines which are restored to fertility by the Rf gene, compared to CMS Petunia lines. An additional transcript with a 5' terminus within the urf-s region of pcf is much more abundant in immature bud and anther tissue than in leaf or suspension cells. The total abundance of pcf transcripts varies greatly between plants of different nuclear backgrounds which lack the nuclear Rf allele, indicating that other nuclear genes also influence expression of the S-pcf locus.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002265335

Download citation: RISBibTeXText

PMID: 1865874

DOI: 10.1007/bf00273922

Related references

Differential Mitochondrial Electron Transport through the Cyanide-Sensitive and Cyanide-Insensitive Pathways in Isonuclear Lines of Cytoplasmic Male Sterile, Male Fertile, and Restored Petunia. Plant Physiology 93(4): 1634-1640, 1990

Effects of Petunia cytoplasmic male sterile (CMS) cytoplasm on the development of sterile and fertility-restored P. parodii anthers. American Journal of Botany 81(5): 630-640, 1994

Differences in kinetics of F1-ATPases of cytoplasmic male sterile, maintainer and fertility restored lines of sorghum. Plant Science (Shannon) 130(1): 19-25, 1997

Localization of mitochondrial proteins in tissue prints of cytoplasmic male sterile cm and fertile petunia lines. Journal of Cell Biology 115(3 Part 2): 300A, 1991

Fertility expression of thermo-sensitive genic male sterile genes under the backgrounds of indica cytoplasmic male sterile lines, restorer lines and maintainer lines. Zhongguo Shuidao Kexue 20(5): 487-492, 2006

Pleiotropic effects of a nuclear restorer-of-fertility locus on mitochondrial transcripts in male-fertile and S male-sterile maize. Current Genetics 35(5): 521-526, 1999

Development of thermo-sensitive cytoplasmic male sterile TCMS lines of wheat characterized by complete male sterility at lower-temperatures and partially restored fertility at higher-temperatures. Euphytica 192(3): 393-399, 2013

The nucleotide sequence and transcription of minicircular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet. Nucleic Acids Research 14(23): 9353-9370, 1986

Interaction of the mitochondrial S-Pcf locus for cytoplasmic male sterility in Petunia with multiple fertility-restoration genes in somatic hybrid plants. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 84(7-8): 829-834, 1992

Mitochondrial DNA analysis and reversion to fertility in Beta vulgaris L. male sterile lines. Journal of Genetics and Breeding 47(2): 121-126, 1993

A MADS-box transcription factor related to fertility conversion in male sterile wheat lines. Acta Agronomica Sinica 34(4): 598-604, 2008