Transfer of cytoplasmic male sterility Tokumasu to fertile rape (Brassica napus) via protoplast fusion

Orsini, J.M.; Abel, W.O.

Physiologia Plantarum 82(1): A28


ISSN/ISBN: 0031-9317
Accession: 002265461

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Somatic hybrids with cytoplasmic male sterility (CMS) were produced by using polyethylene glycol to fuse hypocotyl B. napus protoplasts carrying Tokumasu cytoplasm (containing CMS-inducing mitochondria and cold-sensitive chloroplasts of radish) with leaf mesophyll protoplasts of elite swede rape lines. Hybrid cell frequency was about 30% and over 200 hybrid calluses were obtained after 2 months for transfer to differentiation medium. Regenerated shoots will be characterized by analysis of organelle DNA, nuclear DNA and isoenzymes.