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Use of feeder cells to improve barley protoplast culture and regeneration

Use of feeder cells to improve barley protoplast culture and regeneration

Plant Science 85(2): 179-187

Suspension cultures were initiated from immature-embryo-derived calli of Hordeum vulgare L. (cv. Dissa) and Hordeum marinum Huds. In both species embryogenic suspension lines were used as protoplast donors. While H. vulgare protoplasts isolated from the embryogenic suspensions formed few or no colonies without feeding, feeder cultures gave plating efficiencies up to 10% for these protoplasts. Microcalli were recovered from H. marinum protoplasts only in the presence of feeder cells. Further experiments suggested the involvement of two different components, or growth-promoting factors, in the feeding effect, which may be fully exhibited when feeder cells supplement components which the protoplasts themselves are unable to produce to an adequate level. More than 200 green plantlets were regenerated from H. vulgare protoplasts and 150 regenerations have to date been transferred to soil. The first plants have set seed.

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Accession: 002271033

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DOI: 10.1016/0168-9452(92)90114-2

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