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A rapid and efficient method for the extraction of total DNA from mature leaves of the date palm (Phoenix dactylifera L.)

A rapid and efficient method for the extraction of total DNA from mature leaves of the date palm (Phoenix dactylifera L.)

Plant Molecular Biology Reporter 11(4): 317-319

A method is presented for the rapid isolation of high molecular weight DNA from mature leaves of date palm using a CTAB-based buffer, yielding up to 800 micro g of DNA from 1 g of leaf tissues. It is also suitable for DNA extraction from callus or buds from tissue culture. The DNA obtained is a good substrate for at least 17 restriction endonucleases.

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Accession: 002287691

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