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A rapid plaque assay for Japanese encephalitis virus infectivity by the presence of actinomycin D in overlay medium






Tropical Medicine 34(2): 55-62

A rapid plaque assay for Japanese encephalitis virus infectivity by the presence of actinomycin D in overlay medium

An improved rapid plaque assay for Japanese encephalitis (JE) virus infectivity was developed. When Actinomycin D was introduced at 0.2 mu-g/ml concentration into the overlay medium which contained 2% fetal calf serum and 1% methyl cellulose in Eagle's medium, clear plaques of Nakayama strain JE virus were formed 52 hr postinfection on BHK21 cells. The improved method provided reasonably rapid result of virus infectivity with less labor and cost than conventional plaque or focus assay.

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Accession: 002287764



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