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A successful transfer of exogenous DNA into chickens by microinjection into the germinal disc of fertilized ovum


Proceedings, 19th World' s Poultry Congress, Amsterdam, 19-24 September 1992: Volume 1: 505-508
A successful transfer of exogenous DNA into chickens by microinjection into the germinal disc of fertilized ovum
Plasmid DNA containing the E. coli beta -galactosidase (lacZ) gene under the control of fowl beta -actin gene promoter was injected in linearized from into the germinal disc of fertilized fowl ova at the single-cell stage, obtained from the magnum. The embryos were cultured in vitro and then in surrogate eggshells to hatching. The rate of hatching was 11.08% (31/263); 19 mature birds were obtained.

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Accession: 002289938



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