+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Aberrant reproductive phenotypes evident in transgenic mice expressing the wild-type mouse estrogen receptor

Aberrant reproductive phenotypes evident in transgenic mice expressing the wild-type mouse estrogen receptor

Endocrinology 135(1): 379-386

The estrogen receptor (ER) acts as a transcription factor to regulate multiple cellular functions involved in normal physiology, differentiation, and reproduction. To date, there is no known animal model for studying aberrant ER expression. Therefore, we created transgenic mice expressing the wild-type mouse ER under the control of the mouse metallothionein-I (MT) promoter to determine whether overexpression of the ER would disrupt normal reproductive processes. Five male and one female founder mice were produced, and all were fertile. The progeny from these mice were screened for MT-mER expression by the ribonuclease protection assay. Mice in all six lines were found to express the transgene in a variety of tissues, although generally at low levels. The highest level of expression was observed in the female reproductive tract of line E. Females in all six lines demonstrated aberrant reproductive phenotypes involving processes at parturition and, with some of the lines, a tendency toward reduced fertility. Gestational length was prolonged up to 4 days beyond the normal gestation of 19 days, providing evidence of delayed parturition. In addition, prolonged labor (up to 3 days in length to deliver all pups) and labors requiring cesarean sections for maternal survival demonstrated the occurrence of dystocia in the MT-mER females. As maternal age increased, the incidence of stillborn litters, delayed parturition, and dystocia approached 100% in the transgenic dams. Difficulties at parturition were not observed in nontransgenic control females. These phenotypes suggest that the mechanisms regulating parturition may be perturbed by improper expression of the ER. The MT-mER transgenic mice may provide a novel approach for studying the estrogen-regulated signals involved in parturition and fertility as well as a unique animal model for the human reproductive phenotypes of delayed parturition and dystocia.

(PDF emailed within 0-6 h: $19.90)

Accession: 002290978

Download citation: RISBibTeXText

PMID: 8013372

DOI: 10.1210/endo.135.1.8013372

Related references

Study of 5-hydroxymethylfurfural and its metabolite 5-sulfooxymethylfurfural on induction of colonic aberrant crypt foci in wild-type mice and transgenic mice expressing human sulfotransferases 1A1 and 1A2. Molecular Nutrition and Food Research 56(4): 593-600, 2012

Roles of vasoactive intestinal peptide (VIP) in the expression of different immune phenotypes by wild-type mice and T cell-targeted type II VIP receptor transgenic mice. Journal of Immunology 170(1): 308-314, 2002

Roles of Vasoactive Intestinal Peptide (Vip) in the Expression of Different Immune Phenotypes by Wild-Type Mice and T Cell-Targeted Type Ii Vip Receptor Transgenic Mice. The Journal of Immunology 170(1): 308-314, 2003

Transcription and translation of estrogen receptor-beta in the male reproductive tract of estrogen receptor-alpha knock-out and wild-type mice. Endocrinology 139(6): 2982-2987, 1998

Aberrant phenotypes of transgenic mice expressing dimeric human erythropoietin. Reproductive Biology and Endocrinology 10: 6, 2012

Gene expression profiles with activation of the estrogen receptor alpha-selective estrogen receptor modulator complex in breast cancer cells expressing wild-type estrogen receptor. Cancer Research 62(15): 4419-4426, 2002

Accelerated onset of uterine tumors in transgenic mice expressing a dominant negative variant of the mouse estrogen receptor after neonatal treatment with diethylstilbestrol. Proceedings of the American Association for Cancer Research Annual Meeting 38(0): 434, 1997

The effect of aging on the subcellular distribution of estrogen receptor-alpha in the cholinergic neurons of transgenic and wild-type mice. European Journal of Neuroscience 21(5): 1437-1442, 2005

Immortalized testis cell lines from estrogen receptor (ER) alpha knock-out and wild-type mice expressing functional ERalpha or ERbeta. Journal of Andrology 22(4): 652-664, 2001

Characterization of transgenic mice with conditional over-expression of estrogen receptor alpha and rescue of reproductive phenotype of estrogen receptor alpha knockout mice. Biology of Reproduction 66(Supplement 1): 247, 2002

Imaging of estrogen receptor alpha neurons in transgenic mice expressing GFP under the control of estrogen receptor alpha promoter. Society for Neuroscience Abstracts 27(1): 1081, 2001

Reproductive phenotypes in the estrogen receptor-alpha knockout mouse. Annales D'endocrinologie 60(2): 143-148, 1999

Transgenic tobacco plants co-expressing Agrobacterium iaa and ipt genes have wild-type hormone levels but display both auxin- and cytokinin-overproducing phenotypes. Plant Journal 23(2): 279-284, 2000

Generation and reproductive phenotypes of mice lacking estrogen receptor b. Proceedings of the National Academy of Sciences of the United States of America 95(26): 677-82, 1998

Tissue Distribution and Quantitative Analysis of Estrogen Receptor- (Er) and Estrogen Receptor- (Er) Messenger Ribonucleic Acid in the Wild-Type and Er-Knockout Mouse. Endocrinology 138(11): 4613-4621, 1997