+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Accumulation of hexoses in leaf vacuoles: studies with transgenic tobacco plants expressing yeast-derived invertase in the cytosol, vacuole or apoplasm

Accumulation of hexoses in leaf vacuoles: studies with transgenic tobacco plants expressing yeast-derived invertase in the cytosol, vacuole or apoplasm

Planta 194(1): 29-33

The subcellular distribution of hexoses, sucrose and amino acids among the stromal, cytosolic and vacuolar compartments was analyzed by a nonaqueous fractionation technique in leaves of tobacco (Nicotiana tabaccum L.) wild-type and transgenic plants expressing a yeast-derived invertase in the cytosolic, vacuolar or apoplasmic compartment. In the wild-type plants the amino acids were found to be located in the stroma and in the cytosol, sucrose mainly in the cytosol and up to 98% of the hexoses in the vacuole. In the leaves of the various transformants, where the contents of hexoses were greater than in wild-type plants, again 97-98% of these hexoses were found in the vacuoles. It is concluded that leaf vacuoles contain transporters for the active uptake of glucose and fructose against a high concentration gradient. A comparison of estimated metabolite concentrations in the subcellular compartments of wild-type and transformant plants indicated that the decreased photosynthetic capacity of the transformants is not due to an osmotic effect on photosynthesis, as was shown earlier to be the case in transformed potato leaves, but is the result of a long-term dedifferentiation of tobacco leaf cells to heterotrophic cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002291787

Download citation: RISBibTeXText

DOI: 10.1007/bf00201031

Related references

Transgenic tobacco plants expressing yeast-derived invertase in either the cytosol, vacuole or apoplast: a powerful tool for studying sucrose metabolism and sink/source interactions. Plant Journal 1(1): 95-106, 1991

Solute accumulation and decreased photosynthesis in leaves of potato plants expressing yeast-derived invertase either in the apoplast, vacuole or cytosol. Planta 202(1): 126-136, 1997

Cell wall properties of transgenic tobacco plants that express a yeast derived acid invertase in their vacuole. Visser, J, Voragen, A G J Progress in Biotechnology; Pectins and pectinases: 667-671, 1996

Estimation of the anatomical, stomatal and biochemical components of differences in photosynthesis and transpiration of wild-type and transgenic (expressing yeast-derived invertase targeted to the vacuole) tobacco leaves. Plant, Cell and Environment 20(8): 1070-1078, 1997

The role of sugar accumulation in leaf frost hardiness: Investigations with transgenic tobacco expressing a bacterial pyrophosphatase or a yeast invertase gene. Journal of Plant Physiology 147(5): 604-610, 1996

Expression of a yeast-derived invertase in the cell wall of tobacco and Arabidopsis plants leads to accumulation of carbohydrate and inhibition of photosynthesis and strongly influences growth and phenotype of transgenic tobacco plants. Embo Journal 9(10): 3033-3044, 1990

Analysis of growth, composition and thickness of the cell walls of transgenic tobacco plants expressing a yeast-derived invertase. Protoplasma 200(3-4): 146-153, 1997

Patterns of phenylpropanoids in non-inoculated and potato virus Y-inoculated leaves of transgenic tobacco plants expressing yeast-derived invertase. Phytochemistry 56(6): 535-541, 2001

Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase. Plant & Cell Physiology 42(2): 245-249, 2001

Developmental changes of antioxidative systems in tobacco leaves as affected by limited sucrose export in transgenic plants expressing yeast-invertase in the apoplastic space. Planta 98(2): 253-262, 1996

Correlation between arrested secondary plasmodesmal development and onset of accelerated leaf senescence in yeast acid invertase transgenic tobacco plants. Plant Journal 4(1): 179-189, 1993

"Sink" regulation of photosynthetic metabolism in transgenic tobacco plants expressing yeast invertase in their cell wall involves a decrease of the Calvin-cycle enzymes and an increase of glycolytic enzymes. Planta 183(1): 40-50, 1991

High level accumulation of soybean glycinin in vacuole-derived protein bodies in the endosperm tissue of transgenic tobacco seed. Plant Science (Limerick) 111(1): 39-49, 1995

Apoplastic expression of a yeast derived invertase in transgenic plants leads to dramatic changes in habitus and growth. Journal of Cellular Biochemistry Supplement (14 PART E): 360, 1990

Copper accumulation in transgenic plants over-expressing a yeast metallothionein. Plant Biology (Rockville) : 95, 1998