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Altered nuclear, mitochondrial and plastid gene expression in white barley cells containing ribosome-deficient plastids



Altered nuclear, mitochondrial and plastid gene expression in white barley cells containing ribosome-deficient plastids



Brennicke, A. , Kuech, U. Plant Mitochondria: With Emphasis On Rna Editing And Cytoplasmic Male Sterility: 207-219



The progeny of the barley mutant line albostrians consists of green, white and green-white striped seedlings. Cells from white tissue contain plastids which lack ribosomes and, consequently, all proteins encoded in plastid DNA. In spite of this drastic defect, cells of white leaves have plastids and plastid DNA in quantities comparable to green leaves. Plastid genes for ribosomal proteins (rps2, rps15) and subunits of a putative RNA polymerase (rpoA, rpoB, rpoC1) are transcribed and the mRNAs accumulate to a distinctly higher level (rps15, rpoB/C1) in white compared to green leaves. Genes encoding chloroplast proteins involved in bioenergetic functions (psbA, rbcL, atpI-H), and also tRNA(Glu) and plastid rRNAs, showed little or no accumulation of their transcripts. The data provide strong evidence for a plastid RNA polymerase originating entirely from the nucleo-cytoplasmic compartment. Messengers of mitochondrial genes encoding proteins involved in respiration (coxII, coxIII, atpA, atp6, cob) were found to accumulate to a higher level in white vs. green leaves, whereas mitochondrial 18S and 26S rRNAs were distinctly less affected by the presence of mutant, undifferentiated plastids. Nuclear genes which are light-induced and normally specifically expressed in green tissue (rbcS, cab, genes encoding Calvin cycle enzymes) showed an extremely low accumulation of their mRNAs in white leaves due to a reduced rate of transcription. Although at a very low level, the transcription of these genes was still light-inducible, and a circadian rhythm of cab-mRNA accumulation could be observed. The data are discussed in relation to regulatory interactions between the genomes of plant cells.

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