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Analysis of transgenic animal pedigrees using the junction-polymerase chain reaction



Analysis of transgenic animal pedigrees using the junction-polymerase chain reaction



Pcr Methods and Applications 1(3): 208-209



A polymerase chain reaction (PCR) procedure is described for identifying and screening transgenic animals and their progeny. In animals carrying more than 1 copy of a transgene, the junction between copies represents a unique DNA fragment that can be the target of junction-PCR analysis using appropriately positioned primers.

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Accession: 002301031

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PMID: 1472943



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