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Analysis of whole-cell proteins of Streptococcus thermophilus by 2 electrophoretic methods



Analysis of whole-cell proteins of Streptococcus thermophilus by 2 electrophoretic methods



Lait 74(1): 13-21



The protein profiles obtained using 2 electrophoretic methods were used to compare strains of Streptococcus thermophilus. Whole-cell soluble proteins were extracted and separated by 10-28% gradient PAGE and pH 2.9-9.2 isoelectric focusing. Electrophoretic migration of proteins from 8 strains of S. thermophilus produced highly resolved and reproducible patterns with 30-40 distinct bands using SDS-PAGE and 20-25 bands using isoelectric focusing.

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Accession: 002301075

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