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Anatomical observations on the effects of sucrose concentration on the ontogeny of somatic embryos in maize



Anatomical observations on the effects of sucrose concentration on the ontogeny of somatic embryos in maize



Technical Communication Department of Agricultural Development, South Africa ( 232): 86-90



Immature embryos of the maize inbred line 21A-6 were cultured on a Murashige & Skoog (1962) medium to which 0.5, 1.0 or 2.0 mg 2,4-D/litre and 2 or 6% sucrose had been added. The ontogeny of somatic embryos was determined by the sucrose content in the medium. A 2% sucrose concentration in the initiation medium resulted in somatic embryos that originated from the epidermal and subepidermal cell layers of the coleorhizal end of the scutellum of the cultured immature embryo.

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Accession: 002301253

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