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Androgenesis in 5 cultivars of Citrus limon L. Burm. f

Androgenesis in 5 cultivars of Citrus limon L. Burm. f

Acta Horticulturae ( 300): 315-324

Uninucleate stage anthers of cultivar Femminelli lines L. CNR 8, 9 and 30, Eureka line L. CNR 10, and Monachello line L. CNR 26 were collected from the field in March 1989. After 4 days at 4 degrees C anthers were cultured on 4 media (MS and Chaturverdi & Mitra basic supplemented with 4 or 6% sucrose and 4 combinations of growth regulators). Cultures were transferred to 3500 lux, 16 h photoperiod after one week in the dark at 27 degrees C.

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