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Attempts to develop a transformation system in Agaricus bisporus, utilizing particle bombardment and several other novel approaches

Attempts to develop a transformation system in Agaricus bisporus, utilizing particle bombardment and several other novel approaches

Cultivated Mushroom Research CMR Newsletter 1(1): 11-16

Particle bombardment strategies are described including antibiotic selection using hygromycin B, G418, kanamycin and phleomycin, and bombardment of both mycelia and protoplasts from homokaryons. A number of false-positives were identified, but no transformation was confirmed by Southern hybridization. Transformation of the ascomycete, Ophiastoma ulmi, was used as a positive control.

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Accession: 002306736

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