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Attempts to develop a transformation system in Agaricus bisporus, utilizing particle bombardment and several other novel approaches

Cultivated Mushroom Research CMR Newsletter 1(1): 11-16

Attempts to develop a transformation system in Agaricus bisporus, utilizing particle bombardment and several other novel approaches

Particle bombardment strategies are described including antibiotic selection using hygromycin B, G418, kanamycin and phleomycin, and bombardment of both mycelia and protoplasts from homokaryons. A number of false-positives were identified, but no transformation was confirmed by Southern hybridization. Transformation of the ascomycete, Ophiastoma ulmi, was used as a positive control.

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Accession: 002306736

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