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Baculoviruses: high-level expression in insect cells

, : Baculoviruses: high-level expression in insect cells. Current Opinion in Genetics and Development 3(1): 97-101

Recent developments in the use of baculoviruses as vectors for the expression of eukaryote genes in insect cells are reviewed.

Accession: 002308036

PMID: 8453280

DOI: 10.1016/S0959-437X(05)80348-X

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Related references

Ayola, B.; Kanda, P.; Lanford, R.E., 1993: High level expression and phosphorylation of hepatitis B virus polymerase in insect cells with recombinant baculoviruses. The hepatitis B virus polymerase open reading frame, as well as various subdomains of polymerase, was expressed in insect cells using the recombinant baculovirus expression system. Full-length polymerase was expressed at very low levels in a Spodo...

Keil, Günther.M.; Klopfleisch, C.; Giesow, K.; Blohm, U., 2009: Novel vectors for simultaneous high-level dual protein expression in vertebrate and insect cells by recombinant baculoviruses. Gene transfer into cells of mammalian, avian or piscine origin by baculoviruses carrying expression cassettes active in vertebrate cells (BacMam method) is an attractive alternative to chemical or physical transfection methods or to the use of vec...

Vaughn, J.; Hackett, K.; Deming, C., 2002: Selected insect cell line clones providing increased yield of baculoviruses and gene expression products from recombinant baculoviruses. Three new insect cell lines have been established and characterized. The cloned cell lines are derived from IPLB-Sf-21AE and can grow in serum-free medium. When infected with baculovirus, the cell lines of the invention produce large quantities of...

Miller, L.K., 1988: Baculoviruses for foreign gene expression in insect cells. Insect baculovirus vector systems are rapid and convenient means of obtaining substantial quantities of many different foreign gene products. The vector systems are proving useful in academic research and in industry. Numerous examples of the succ...

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Atkinson A.E.; Weitzman M.D.; Obosi L.; Beadle D.J.; King L.A., 1990: Baculoviruses as vectors for foreign gene expression in insect cells. Recombinant DNA technology has enabled the expression of a large number of eukaryotic genes in vitro. There has been growing interest in recent years in the development of baculovirus expression vectors as an easily manipulated gene expression sys...

Klein, R.; Schröder, C.H.; Zentgraf, H., 1991: Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses. The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear p...

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