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Basic EDTA as an extractant for soil organic phosphorus






Soil Science Society Of America Journal. 57(6): 1516-1518

Basic EDTA as an extractant for soil organic phosphorus

Soil organic P is an important source of plant-available P in the Great Plains. It is, therefore, important in conservation tillage agriculture, that we know the size and the potential availability for crop use of this large P pool, which could exceed one-half of the total P. We propose a one-step procedure with hot basic EDTA (ethylene diaminetetraacetic acid) for total soil organic P that requires minimum manipulation and analytical prowess. Soil samples (0.5 g) were incubated for 2 h at 85 degrees C with 25 mL of 0.25 M NaOH + 0.05 M Na2EDTA, after which time the organic P in the extracts was determined by persulfate oxidation. Results from the evaluation of nine soils from various parts of the USA and one Canadian soil showed significant correlations with an existing wet sequential extraction and a dry high-temperature ignition method for total organic P determinations. With the high-organic-matter Canadian soil, the EDTA method extracted 32% more organic P than the wet extraction method, and essentially the same as the dry ignition method. The new method appears to be especially useful for soils where organic acids and chelation mechanisms with P may be more dominant than ester-type formations.

Accession: 002308377

DOI: 10.2136/sssaj1993.03615995005700060020x

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Related references

Martin, J.K., 1964: Soil organic phosphorus : I. Methods for the extraction and partial fractionation of soil organic phosphorus. II. The nature of soil organic phosphorus. III. Patterns of organic phosphorus in a series of representative New Zealand soils. Extraction of organic P from a black loam by several acidic and alkaline reagents was investigated. The proportion of total organic P extracted by acid solutions varied from 3 % with 0.1 n HC1 to 85 % with 10 n H2SO4. There was evidence for some d...

Van Lierop W., 1989: Effect of edta and dtpa on available phosphorus extraction with the kelowna multiple element extractant. The objective of this study was to determine the effect of adding either 0.001M EDTA or 0.005M DTPA on the amount of P removed from acid and calcareous soils by the Kelowna and 0.25M HOAc extractants. These complexing agents were studied for possi...

Harrap, F.E.G., 1963: Use of sodium EDTA in the determination of soil organic phosphorus. The organic phosphorus contents of a range of soils were determined by the extraction method of Mehta et al. (1954) and by the ignition method of Legg and Black(1955) and the results compared with those obtained by an extraction method using Na2 E...

McKeague, J.A.; Schuppli, P.A., 1985: An assessment of EDTA as an extractant of organic-complexed and amorphous forms of Fe and Al in soils. EDTA solutions proposed as selective extractants of amorphous and organic forms of Fe and Al in soils and synthetic materials were tested. Extraction of Fe and Al from some soil samples continued at a decreasing rate beyond 120 days. For some soil...

Ahmed, B.; Islam, A., 1975: The use of sodium EDTA as an extractant for determining available phosphate in soil. The use of a neutral solution of 0.0025M Na2EDTA as an extractant in the assessment of soil phosphate available to plants is described. In the soil types used, a high correlation was found between EDTA extractable soil phosphate and plant response...

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Pierre, W.H.; Parker, F.W., 1927: Soil phosphorus studies: II. The concentration of organic and inorganic phosphorus in the soil solution and soil extracts and the availibility of the organic phosphorus to plants. Soil Science 24(2): 119-128

Anonymous, 1927: Soil phosphorus studies II The concentration of organic and inorganic phosphorus in the soil solution and soil extracts and the availability of the organic phosphorus to plants

Beyers, C.P.D.L.; Coetzer, F.J., 1971: Effect of concentration, pH and time on the properties of di-ammonium EDTA as a multiple soil extractant. Di-ammonium EDTA at concentrations between 0.01 to 0.05 M at pH 4.6 to 9.5 was used as an extractant for 30, 60 or 120 min for Cu, Zn, Mn, Fe, AI, B, Ca, K and Mg from soil samples of widely varying clay content (0.9 to 40%). Increasing EDTA conce...

Ramos, B.M., 1950: The aim of determination of assimilable phosphorus in the soil. Introduction to the study of the influence of the pH of the extractant on the content of phosphorus extracted.. The Truog-Meyer method was used, with extractant solutions consisting of various dilutions of H2SO4 buffered to pH values of I, 2, 3, 4, 5 and 6 by means of (NH4)2SO4.