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Basidiospore viability and germination in ectomycorrhizal and saprotrophic basidiomycetes

Basidiospore viability and germination in ectomycorrhizal and saprotrophic basidiomycetes

Mycological Research 97(2): 141-149

Spores of the ectomycorrhizal basidiomycetes Rhizopogon rubescens an Suillus tomentosus were induced to germinate and stained with fluorescent strains FDA and DAPI to assess germinability and viability. Basidospores of several saprotrophic species including Pleurotus ostreatus, Marasmius oreades, Agaricus brunnescens, Coprinus quadrifidus and Conocybe lactea were also assayed for comparison. Nearly all spores of both the ectomycorrhizal and saprotrophic fungi contained intact nuclei. The percentage of R. rubescens and S. tomentosus spores that stained with FDA increased throughout the study. Peaks in FDA staining corresponded with germination events which occurred every 9 to 13 days. The FDA staining in spores of P. ostreatus and M. oreades was high and corresponded with high rates of germinability. Germination and FDA staining of the other saprotrophic species were variable. In ectomycorrhizal and several saprotrophic species FDA staining was unreliable as an indicator of viability but served as a good predictor of dormancy. Non-fluorescent vital and nuclear stains were compared with the fluorescent stains and gave similar results. Equations for estimating viability, dormancy, and activation of spores are presented.

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Accession: 002308470

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DOI: 10.1016/s0953-7562(09)80235-9

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