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Basidiospore viability in stored slurries

Basidiospore viability in stored slurries

Mycological Research 98(5): 527-530

The viability and activity of basidiospores corresponding to 14 species of ectomycorrhizal fungi stored in slurries was studied. The methods used were the aceto-iron-haematoxylin nuclear stain to determine basidiospore viability and the FDA stain to determine basidiospore activity and dormancy. The basidiospores of Cortinarius sp., Hebeloma sp., Inocybe sp., Laccaria bicolor, Laccaria laccata, Lactarius deliciosus, Lactarius sanguifluus, Rhizopogon roseolus, Russula delica, Russula sanguinea, Suillus collinitus, Suillus granulatus, Suillus variegatus and Tricholoma terreum were suspended in slurries in duplicate experiments and half were stored at 3-4 degree C and the other half at -15 degree . Spore viability and activity was tested after 180 days of storage. Loss of viability was least in species of Rhizopogon and Suillus, but data showed an almost total loss of viability in both refrigerated and frozen slurries of all fungi.

Accession: 002308471

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DOI: 10.1016/S0953-7562(09)80473-5

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