+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Behavior of pea nodulation mutants as affected by increasing nitrate level

Symbiosis 15(3): 195-206
Behavior of pea nodulation mutants as affected by increasing nitrate level
Nodule number and size, acetylene-reducing activity, leghemoglobin content in nodules, and nitrogen content in the shoot were measured in pea symbiotic mutants inoculated with Rhizobium leguminosarum bv. viceae strain 128C30 and cultivated on a range of nitrate levels in rooting medium. The abundantly nodulating mutants RisfixC and RisfixV, and the ineffective mutant RisfixO were compared to the parental cultivar 'Finale'. RisfixO displayed nodule initiation and nodule growth suppression similar to the wild-type plants, indicating that nodule effectiveness is not substantial for both endogenous and nitrate regulation of modulation. RisfixC was seen to produce effective and nitrate-resistant modulation in abundance, confirming its classification as a new supernodulating pea mutant. The nitrate resistance was apparent not only in nodule initiation, but also in nodule growth and specific nitrogenase activity. Shoot nitrogen content was markedly increased in RisfixC, indicating carbohydrate depletion or a regulatory fault. Nodulation in RisfixV was abundant like in RisfixC, but the nodules possessed only traces of nitrogenase activity, and their number sharply diminished with nitrate level increase. The small and nitrate-independent size of RisfixV nodules suggests that their development had been arrested before nitrate level affected nodule growth. The hypernodulation of RisfixV is supposed to result from the early deterioration of nodules of this mutant, which releases the suppression of further nodule initiation by the established nodules.

Accession: 002308805

Related references

Nitrogen fixation of nodulation mutants of soybean as affected by nitrate. Plant Physiology 92(4): 1142-1147, 1990

Nodulation, acetylene reduction and yield of faba beans as affected by inoculum concentration and soil nitrate level. Canadian Journal of Plant Science 57(4): 1055-1061, 1977

A Lotus japonicus mutant defective in nitrate uptake is also affected in the nitrate response to nodulation. Plant Biology 17(1): 16-25, 2016

Nitrate effects on the nodulation of legumes inoculated with nitrate-reductase-deficient mutants of Rhizobium. Planta 134(1): 17-22, 1977

Nitrate inhibition of nodulation and nitrogen fixation by supernodulating nitrate-tolerant symbiosis mutants of soybean. Crop science 29(6): 1491-1496, 1989

Nitrate effects on the nodulation of legumes inoculated with nitrate reductase deficient mutants of rhizobium trifolii. Planta (Heidelberg) 134(1): 17-22, 1977

Effects of nitrate application on growth nodulation and nitrogen fixation of nitrate tolerant mutants of soybean. Soil Science & Plant Nutrition 37(3): 521-530, 1991

Nitrate inhibits nodulation of supernodulating soybean mutants. Gresshoff, P M , Et Al (Ed ) Nitrogen Fixation: Achievements And Objectives; 8th International Congress on Nitrogen Fixation, Knoxville, Tennessee, Usa, May 20-26, Xix+869p Routledge, Chapman And Hall: New York, New York, Usa; London, England, Uk Illus 734, 1990

Mutants of rhizobium meliloti affected in nodulation ability. Acta Microbiologica Polonica 38(1): 5-16, 1989

Effect of nitrate on nodule activity of soybean nodulation mutants. Plant Physiology (Rockville) 89(4 SUPPL): 15, 1989