Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons
Teramoto, H.; Momotani, E.; Tsuji, H.
Physiologia Plantarum 87(4): 584-591
1993
ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1993.tb02510.x
Accession: 002309251
Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons were studied. Poly (A)+ RNA was prepared from etiolated cotyledons incubated with or without benzyladenine (BA) for various periods in the dark. Using nonequilibrium pH gradient electrophoresis-SDS polyacrylamide gel electrophoresis and isoelectric focusing-SDS polyacrylamide gel electrophoresis, both basic and neutral proteins translated in vitro were separated. About 240 spots were detected and 16 of them changed within 6 h after BA application. Some spots changed quickly (within 1-2 h). Among them, three were repressed markedly.