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Changes in hemolymph of the migratory grasshopper, Melanoplus sanguinipes, infected with an entomopoxvirus



Changes in hemolymph of the migratory grasshopper, Melanoplus sanguinipes, infected with an entomopoxvirus



Journal of Invertebrate Pathology 60(3): 274-282



Hemolymph changes in response to infection with Melanoplus sanguinipes entomopoxvirus (MsEPV) were studied in the migratory grasshopper, M. sanguinipes. Second-instar grasshoppers were inoculated orally with MsEPV (5.0 times 10-3 occlusion bodies (OBs)/individuals) and hemolymph was collected at specified intervals from 0 to 32 days post-inoculation (p.i.). Mature OBs were detected in hemolymph samples from Day 21 p.i. onward. Total hemocyte counts decreased in infected verus control grasshoppers after 10-15 days p.i. Phenoloxidase activity initially peaked at Day 4 p.i. in infected grasshoppers and then declined to control levels until late in infection. Hemocytes from infected individuals on Day 28 p.i. showed a five- to sixfold increase in binding of fluorescein-isothiocyanate-labeled wheat germ agglutinin. Hemocyte morphological changes were observed late in infection including a small percentage of enlarged hemocytes and cells with extensive pseudopodia. MsEPV OBs were also observed in the cytoplasm of hemocytes late in infection. Whether the changes noted in M. sanguinipes hemolymph were a direct result of virus infection or a physiological response secondary to infection is discussed.

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Accession: 002318429

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DOI: 10.1016/0022-2011(92)90009-s


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