Comparison of fertility between the F1, F2 and anther derived lines in the crosses of indica/japonica and japonica/indica in rice (Oryza sativa L.)

Narasimman, R.; Rangasamy, S.R.

Euphytica 66(1-2): 19-25

1993


ISSN/ISBN: 0014-2336
DOI: 10.1007/bf00023504
Accession: 002328350

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Abstract
Response of anthers in in vitro culture was examined in indica-japonica hybrids of rice (Oryza sativa). Significant genotypic differences were observed for callus induction and regeneration among the different interracial hybrids of indica-japonica races. Induction frequency of haploids ranged from 57.7 to 72.9% and doubled haploid androgenic lines ranged from 27.1 to 42.3% in the anther culture of the different hybrids. The indica-japonica hybrids recorded partial pollen grain and spikelet fertility in F1 (29.9 to 41.5% and 19.4 to 48.7%, respectively) as well as in F2 (42.7 to 50.6% and 37.1 to 54.4%, respectively). In contrast, the androgenic doubled haploid lines recorded a significant increase and the pollen grain and spikelet fertility was 76.3 and 78.6%, respectively. The results suggested that the sterility barriers for realising genetic recombinants and fixation of fertile homozygous lines in an indica-japonica hybridization programme could be overcome through F1 anther culture technique.

Comparison of fertility between the F1, F2 and anther derived lines in the crosses of indica/japonica and japonica/indica in rice (Oryza sativa L.)