EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Detection of strains of potato virus S by nucleic acid spot hybridization (NASH)


Detection of strains of potato virus S by nucleic acid spot hybridization (NASH)



Potato Research 33(4): 487-495



ISSN/ISBN: 0014-3065

DOI: 10.1007/bf02358027

Two complementary DNA (cDNA) clones (0.9 kb and 1.6 kb) reacting to the ordinary strain of potato S carlavirus (PSV) were compared with single-stranded randomly primed cDNA (prepared to total genomic RNA) as probes for various strains of PSV, using nucleic acid spot hybridization (NASH). The cDNA clones detected 11 PSV isolates well, including both Andean and ordinary strains, while one of each strain was detected weakly. As little as 5 femtograms of PSV RNA could be detected.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002341267

Download citation: RISBibTeXText

Related references

Detection of strains of potato virus x and of a broad spectrum of potato virus y isolates by nucleic acid spot hybridization nash. Plant Disease 72(4): 307-309, 1988

Multiplex detection of Potato virus S, Potato virus X, and Potato Virus Y by non-radioactive nucleic acid spot hybridization in potato tissue culture plantlets. American Journal of Potato Research 83(6): 495-501, 2006

Detection of potato leafroll virus by nucleic acid spot hybridization with 32P labelled cDNA probe. Virologica Sinica 10(1): 75-80, 1995

Detection of Andean potato virus X isolates by radioactive and nonradioactive nucleic acid spot hybridization tests. Phytopathology 83(2): 171-176, 1993

Detection of sweetpotato chlorotic stunt crinivirus (SPCSV) by non-radioactive Nucleic Acid Spot Hybridization (NASH) technique. Acta Horticulturae (583): 129-133, 2002

Detection of potato spindle tuber viroid by nucleic acid spot hybridization: evaluation with tuber sprouts and true potato seed. American Potato Journal 60(8): 587-597, 1983

Comparative detection of mild strains of potato spindle tuber viroid from the dormant potato tubers by return-polyacrylamide gel electrophoresis and nucleic acid hybridization. Potato Research 31(1): 159-166, 1988

Detection of epstein barr virus dna in lymphoid tissues by nucleic acid spot hybridization. Pediatric Research 16(4 Part 2): 234A, 1982

Biotinylated nucleic acid hybridization probes for potato virus detection. Archives of Virology 103(3-4): 231-241, 1988

Development of a nucleic acid hybridization assay for detection of a closterovirus associated with the sweet potato virus disease complex. Phytopathology 82(10): 1144, 1992

The sensitivity and specificity of a rapid nucleic acid hybridization method for the detection of potato virus x in crude sap samples. Plant Pathology 33(3): 361-370, 1984

Nucleic acid spot hybridization for detection of chlamydia trachomatis. FEMS Microbiology Letters 28(1): 85-91, 1985

Comparison of four radioactive probes for the diagnosis of potato spindle tuber viroid by nucleic acid spot hybridization. Potato Research 31(3): 431-442, 1988

Detection of adenoviruses in stool specimens by nucleic acid spot hybridization. Journal of Medical Virology 16(3): 213-218, 1985

Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus. Journal of Clinical Microbiology 31(4): 986-989, 1993