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Detection of strains of potato virus S by nucleic acid spot hybridization (NASH)

Detection of strains of potato virus S by nucleic acid spot hybridization (NASH)

Potato Research 33(4): 487-495

ISSN/ISBN: 0014-3065

DOI: 10.1007/bf02358027

Two complementary DNA (cDNA) clones (0.9 kb and 1.6 kb) reacting to the ordinary strain of potato S carlavirus (PSV) were compared with single-stranded randomly primed cDNA (prepared to total genomic RNA) as probes for various strains of PSV, using nucleic acid spot hybridization (NASH). The cDNA clones detected 11 PSV isolates well, including both Andean and ordinary strains, while one of each strain was detected weakly. As little as 5 femtograms of PSV RNA could be detected.

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Accession: 002341267

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