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Effect of enzymatic hydrolysis on the morphology and fine structure of pretreated cellulosic residues


, : Effect of enzymatic hydrolysis on the morphology and fine structure of pretreated cellulosic residues. Enzyme & Microbial Technology 15(10): 821-831

The action of Trichoderma cellulases on the various characteristics of cellulosic fractions obtained from pretreated eucalyptus chips was investigated. A screened fraction derived from a fully bleached eucalyptus kraft pulp (FBEP-48 fraction) was shown to consist of fibers with an average fiber length and an average degree of polymerization (DP) considerably higher than that obtained for the screened fraction derived from SO-2-impregnated steam-exploded eucalyptus chips which had been posttreated with alkaline hydrogen peroxide (SEE-H-2O-2, F-150 fraction). Both fractions were shown to be readily and effectively hydrolyzed by the cellulases present in the Trichoderma Celluclast preparation (Novo Nordisk). The process of enzymatic hydrolysis of both FBEP-48 and F-150 fractions resulted in a rapid decrease in the length distribution of the fibers, while small particles rapidly accumulated in the hydrolysis mixture. These particles were subsequently degraded to equivalent amounts of soluble sugars. Glucose was the main hydrolysis product, due to high amount of beta-glucosidase activity which had been added. However, at the molecular level, it was apparent that the two cellulosic substrates were hydrolyzed in distinct ways and that the mechanism appeared to be influenced by the fine structure of the substrate. As hydrolysis resulted in a gradual decrease in both the degree of polymerization and the degree of crystallinity of the F-150 fraction, it was probable that the depolymerization of this substrate was predominantly due to exoglucanase activity. By contrast, the enzymatic hydrolysis of the FBEP-48 fraction resulted in little change in either the cellulose DP or the degree of crystallinity of the substrate. The suggested a "peeling off" type of mechanism. The susceptibility of the pretreated substrates to enzymatic hydrolysis could not be easily predicted from the differences in their cellulose DP or crystallinity.

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Accession: 002355404

DOI: 10.1016/0141-0229(93)90093-h

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Li, X. J., 1995: A study on the comprehensive exploitation and utilization of chicken manure resources. The use of treated chicken manure, especially dried chicken manure, as a feed and fertilizer is considered. The environmental effects of chicken farms are discussed. Research showed that dried chicken manure can safely be used as a feed and fertil...

Dabrowski M., 1970: The role of thymus in the immunological response of the draining lymph node in rats with adjuvant induced poly arthritis part 2 auto radiographic investigations on immunological reactivity of the lymphoid cells. Polish Medical Journal (English Translation of Polskie Archiwum Medycyny Wewnetrznej) TT(70-55007-4): 908-913

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Chaieb, M.; Floc' h, E.L.; Pontanier, R., 1996: C3 and C4 plants: competition or complementarity? I: growth and phenology. In the arid zone of Tunisia, Stipa lagascae (C3) and Cenchrus ciliaris (C4), were planted in pure and mixed stands in lysimetric cases in order to compare the growth and phenology of the two biochemically contrasted species. When planted together,...

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Anding, K.; Rost, J.M.; Jacobs, E.; Daschner, F.D., 2003: Flow cytometric measurements of neutrophil functions: the dependence on the stimulus to cell ratio. Phagocytosis and antimicrobial killing of neutrophils has been quantitatively determined as a function of the stimulus (Candida albicans) to cell ratio R using two donor collectives containing a total of 115 blood samples. Analysis of the collecti...

Lee, Z.; Klaver, J.R.; Hart, S.D.; Moretti, M.M.; Douglas, K.S., 2010: Short-term stability of psychopathic traits in adolescent offenders. There is considerable debate about the assessment of psychopathic traits in adolescence due in part to questions regarding the stability of traits. We investigated the 6-month stability of psychopathic traits in a sample of 83 male adolescent offe...

Breen, J.L.; Peraglie, B.R., 1966: Postmortem cesarean section. Report of a case. Pacific Medicine and Surgery 74(2): 102-104

Schultheisz, F.; Kerényi, K.; Ravasz, G.; Kaposi, P., 1983: Appendicitis causing an unusual combination of clinical symptoms. Orvosi Hetilap 124(7): 407-408

Tetteroo, P.A.; Mulder, A.; Lansdorp, P.M.; Zola, H.; Baker, D.A.; Visser, F.J.; von dem Borne, A.E., 1984: Myeloid-associated antigen 3-alpha-fucosyl-N-acetyllactosamine (FAL): location on various granulocyte membrane glycoproteins and masking upon monocytic differentiation. Seven different granulocyte-reactive murine monoclonal antibodies (mAb) were studied. The antigens recognized by these mAb were immunoprecipitated from lysates of 125I-labeled granulocytes of healthy donors. The isolated antigens were analyzed by...