EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of ethylene and culture environment on rice callus proliferation


, : Effect of ethylene and culture environment on rice callus proliferation. Journal of Experimental Botany 44(269): 1829-1835

Modifications to the gaseous envelope by callus during culture in Petri dishes were shown to reduce growth and promote necrosis of several rice (Oryza sativa L.) cultivars. Incubating callus under a continuous flow of gas mixtures of known composition suggested that the inhibition of growth was caused by the accumulation of ethylene, the depletion of oxygen and, to a lesser extent, the accumulation of carbon dioxide. In order to evaluate the importance of ethylene accumulation aminoethoxyvinylglycine (AVG), 1-aminocyclopropane-1-carboxylic acid (ACC) and silver nitrate (AgNO-3), were added to the nutrient medium and ethylene measurements performed during callus culture. Ethylene restricted callus growth particularly under high (35 degree C) as compared to moderate (25 degree C) temperatures and under illuminated as compared to darkened incubation. Under illuminated incubation at 25 degree C AVG (5 mmol m-3) and AgNO-3 (50 mmol m-3) significantly improved callus growth (100 and 60%, respectively) while ACC (200 mmol m-3) Significantly decreased growth (40%). AVG and AgNO-3 were less effective under dark incubation at 25 degree C where ethylene production was lower. Furthermore, callus growth was significantly better in large as compared to small culture vessels since the ethylene concentration was diluted and more oxygen was available for respiration. Better control of ethylene and increased oxygen availability could be a way of producing healthy callus for the formation of embryogenic tissues of otherwise recalcitrant cultivars of rice (e.g. Indica IR42) and may be a way of improving manipulation of other cereal species.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002355469

DOI: 10.1093/jxb/44.12.1829

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Yoshikura, H., 2014: Which age group is spreading measles in Japan?. Japanese Journal of Infectious Diseases 66(5): 459-462

Michel, R.; Westphal, A., 1969: Die Spezifitat der Dermal- und Peritoneal-Zellreaktion sowie der Eosinotaxis bei der durch Trypanosoma vaginalis sensibilisierten Maus. Zeitschrift fuer Tropenmedizin und Parasitologie, 20: 151-161

Jansson, L.; Ehnevid, H.; Blomlöf, L.; Weintraub, A.; Lindskog, S., 1995: Endodontic pathogens in periodontal disease augmentation. Periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. This has been associated with patency of dentinal tubules in the tooth...

Annas, G.J.; Roche, P.; Green, R.C., 2008: GINA, genism, and civil rights. Bioethics 22(7): Ii-Iv

Dong, L.; He, J.; Li, Q.; Chen, X.; Hu, Z., 2003: Study of the reaction of proteins with 3-hydroxy-4- azo by Rayleigh light scattering technique. This is the first report on the determination of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo (Cal-Red) by Rayleigh light scattering (RLS). At pH 4.07, the weak RLS of Cal-Red can be enhanced greatly by the addition of proteins....

Winemiller K.O., 1989: Development of dermal lip protuberances for aquatic surface respiration in south american characid fishes. Thirteen characid and one gasteropelecid fish species from a seasonal swamp-creek of the Venezuelan Ilanos exhibited dermal protuberances of the lower lip during periods of extreme oxygen depletion. Relative frequencies of protuberances showed lar...

Williams, S.C., 2010: Texas Tech University Health Sciences Center School of Medicine. Academic Medicine 85(9 Suppl): S551-S554

Decottignies, A., 2014: The telomere position effect: silence in the back row!. Heterochromatin displays repressive histone marks that down-regulate transcription. In the absence of specialized barriers, these repressive marks spread onto nearby nucleosomes and induce transcriptional silencing of these regions. Accordingly, i...

Thorne, Gerald, 1929: Nematodes from the summit of Longs Peak, Colorado. About 3000 nematodes were found in 2 lots of soil collected at an elevation of 14,255 feet. Eighteen species, representing 11 genera, were present. Of these, 6 are new species and the remaining 12 well known European forms. The latter are Tylenchu...

Blackman, C. F.; Blanchard, J. P.; Benane, S. G.; House, D. E., 1994: Agreement between IPR model predictions and magnetic field influence on PC-12 cell neurite production. Molecular Biology of the Cell 5(SUPPL ): 359A