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Effect of ethylene and culture environment on rice callus proliferation

, : Effect of ethylene and culture environment on rice callus proliferation. Journal of Experimental Botany 44(269): 1829-1835

Modifications to the gaseous envelope by callus during culture in Petri dishes were shown to reduce growth and promote necrosis of several rice (Oryza sativa L.) cultivars. Incubating callus under a continuous flow of gas mixtures of known composition suggested that the inhibition of growth was caused by the accumulation of ethylene, the depletion of oxygen and, to a lesser extent, the accumulation of carbon dioxide. In order to evaluate the importance of ethylene accumulation aminoethoxyvinylglycine (AVG), 1-aminocyclopropane-1-carboxylic acid (ACC) and silver nitrate (AgNO-3), were added to the nutrient medium and ethylene measurements performed during callus culture. Ethylene restricted callus growth particularly under high (35 degree C) as compared to moderate (25 degree C) temperatures and under illuminated as compared to darkened incubation. Under illuminated incubation at 25 degree C AVG (5 mmol m-3) and AgNO-3 (50 mmol m-3) significantly improved callus growth (100 and 60%, respectively) while ACC (200 mmol m-3) Significantly decreased growth (40%). AVG and AgNO-3 were less effective under dark incubation at 25 degree C where ethylene production was lower. Furthermore, callus growth was significantly better in large as compared to small culture vessels since the ethylene concentration was diluted and more oxygen was available for respiration. Better control of ethylene and increased oxygen availability could be a way of producing healthy callus for the formation of embryogenic tissues of otherwise recalcitrant cultivars of rice (e.g. Indica IR42) and may be a way of improving manipulation of other cereal species.

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Accession: 002355469

DOI: 10.1093/jxb/44.12.1829

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Yoshikura, H., 2014: Which age group is spreading measles in Japan?. Japanese Journal of Infectious Diseases 66(5): 459-462

Michel, R.; Westphal, A., 1969: Die Spezifitat der Dermal- und Peritoneal-Zellreaktion sowie der Eosinotaxis bei der durch Trypanosoma vaginalis sensibilisierten Maus. Zeitschrift fuer Tropenmedizin und Parasitologie, 20: 151-161

Jansson, L.; Ehnevid, H.; Blomlöf, L.; Weintraub, A.; Lindskog, S., 1995: Endodontic pathogens in periodontal disease augmentation. Periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. This has been associated with patency of dentinal tubules in the tooth...

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Blackman, C. F.; Blanchard, J. P.; Benane, S. G.; House, D. E., 1994: Agreement between IPR model predictions and magnetic field influence on PC-12 cell neurite production. Molecular Biology of the Cell 5(SUPPL ): 359A