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Effect of Exogenous Abscisic Acid on Proline Dehydrogenase Activity in Maize (Zea mays L.)


, : Effect of Exogenous Abscisic Acid on Proline Dehydrogenase Activity in Maize (Zea mays L.). Plant Physiology 99(2): 762-764

Plant responses to drought stress include proline and abscisic acid (ABA) accumulation. Proline dehydrogenase (PDH) (EC 1.4.3) is the first enzyme in the proline oxidation pathway, and its activity has been shown to decline in response to water stress (PJ Rayapati, CR Stewart [1991] Plant Physiol 95: 787-791). In this investigation, we determined whether ABA treatment affects PDH activity in a manner similar to drought stress in maize (Zea mays L.) seedlings. Four exogenous ABA treatments (0, 11, 33, and 100 micromolar ABA) were applied to well-watered maize seedlings. Mitochondria were isolated and PDH was solubilized using Nonidet P-40. PDH activity was measured by the reduction of iodonitrotetrazolium violet under proline-dependent conditions. There was no effect of ABA on PDH activity at 33 and 100 micromolar ABA, but there was a 38% decline at 11 micromolar. This decline was less than the 69% reduction in activity under drought stress. Endogenous ABA determinations and plant growth rate showed that ABA entered the plant and was affecting metabolic processes. ABA treatments had a small effect on shoot and root proline concentration, whereas drought stress caused a 220% increase in root tissues. We conclude that ABA is not part of the pathway linking drought stress and decreased PDH activity.

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Accession: 002355503

PMID: 16668952

DOI: 10.1104/pp.99.2.762

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Related references

Ilahi, I.D.rffling, K., 1982: Changes in abscisic acid and proline levels in maize varieties of different drought resistance Zea mays. Physiologia plantarum 55(2): 129-135

Ober, E., S.; Sharp, R., E., 1994: Proline accumulation in maize (Zea mays L.) primary roots at low water potentials: Requirement for increased levels of abscisic acid. Previous work showed that the concentration of proline (Pro) increases greatly in the primary root tip of maize (Zea mays L.) at low water potentials (psi-w). It was also shown that the maintenance of root elongation at low psi-w depends on increa...

Ober, E..S..; Sharp, R..E.., 1994: Proline Accumulation in Maize (Zea mays L.) Primary Roots at Low Water Potentials (I. Requirement for Increased Levels of Abscisic Acid). Previous work showed that the concentration of proline (Pro) increases greatly in the primary root tip of maize (Zea mays L.) at low water potentials. It was also shown that the maintenance of root elongation at low water potentials depends on inc...

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