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Effect of extenders, additives and deep freezing on the leakage of transaminases from Murrah buffalo spermatozoa


, : Effect of extenders, additives and deep freezing on the leakage of transaminases from Murrah buffalo spermatozoa. Buffalo Journal 9(1): 55-64

The effects of diluent (Tris, citrate and lactose), additives (0.1% cysteine, 0.1% EDTA and 1.0% raffinose), and freezing for 24 h on the leakage of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) from Murrah buffalo spermatozoa were studied.

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Related references

Dhami, A.J.; Sahni, K.L., 1993: Effect of extenders, additives and deep freezing on the leakage of lactic dehydrogenase from cattle and buffalo spermatozoa. The effect of extenders (TFYG, EYCG and LYG), additives (0.1% cysteine, 0.1% EDTA and 1.0% raffinose), bulls and stages of freezing on the leakage of lactic dehydrogenase (LDH) enzyme from cattle and buffalo spermatozoa was investigated in 36 ejac...

Jagir Singh; Pangawkar, G.R.; Biswas, R.K.; Shrivastava, A.K., 1991: Studies on leakage of transaminases from buffalo spermatozoa in relation to deep-freezing in certain extenders. Egg yolk-citrate-glycerol, Tris-egg yolk-glycerol and citric acid-whey-glycerol extenders were compared for the leakage of glutamic oxaloacetic transaminase and glutamic pyruvate transaminase from buffalo spermatozoa during different stages of fre...

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Iqbal, N.; Khan, I.H.; Anzar, M.; Choudhary, R.A., 1987: Effect of extenders on the extracellular release of transaminases from deep frozen buffalo spermatozoa. Extracellular release of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) was studied in 50 semen samples collected from 10 Nili-Ravi bulls. Lactose-egg yolk-glycerol (LEG), Tris-egg yolk-glycerol (TEG) and skim milk...

Dhami, A.J.; Sahni, K.L.; Mohan, G., 1995: Effect of various extenders and additives on deep-freezing, enzyme leakage and fertility of bovine semen under tropical climate. The relative merits of tris (TFYG), citrate (EYCG) and lactose (LYG) based diluents without or with inclusion of cysteine (IC) 0.1%, EDTA 0.1% and raffinose 1.0% were evaluated under split-sample technique for deep-freezing of semen of 3 HF bulls...

Rathore, A.K., 1965: Effect of instantaneous deep freezing (-79 degrees C) on survival of buffalo bull (Murrah) spermatozoa with two levels of glycerol. Four semen samples were collected from each of 4 bulls; each sample was diluted in egg yolk-citrate and split into 2 parts. After storage at 5 degrees C for 2 hrs., the 2 parts of each sample were equilibrated for 18 hrs. with 7 or 14% glycerol an...

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