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Effect of follicle cells and steroidogenesis on maturation and fertilization in vitro of pig oocytes


, : Effect of follicle cells and steroidogenesis on maturation and fertilization in vitro of pig oocytes. Journal of Experimental Zoology 266(2): 146-151

Follicle cell numbers, steroidogenesis, and culture conditions were manipulated in a series of experiments designed to identify their during in vitro maturation of pig oocytes upon meiotic progression, sperm penetration and male pronuclear formation. In Experiment 1 a static culture system was used to determine the effect of follicle cell numbers on maturation. When the follicle cell number was 3.5 times 10-3 cells per complex or less, cell cycle progression to metaphase II (M-II) and male pronuclear formation were both low ( lt 30%). Increasing follicle cell numbers to 15 times 10-3 per complex significantly enhanced both meiotic maturation (93%) and male pronuclear formation (83%). A further 10-fold increase in follicle cell numbers (150 times 10-3), however, reduced subsequent male pronuclear formation (33%). The purpose of Experiment 2 was to determine whether the observed follicle cell effects were influenced by the type of culture system used for oocyte maturation. Comparisons were made between both static and non-static forms of culture. Meiotic progression was significantly depressed when non-static culture systems were used for the maturation of oocyte-cumulus complexes containing low numbers of somatic cells ( lt 15 times 10-3 cells). However, the addition of somatic cells (follicle shells) to non-static, but not static, culture systems significantly enhanced male pronuclear formation in all groups irrespective of the number of cumulus cells in the oocyte complex. Experiment 3 investigated the effects of the steroid environment on maturation and male pronuclear formation. The addition of high levels of progesterone (1 mg/ml) from the beginning of maturation was detrimental for maturation. More interestingly, inhibition of steroidogenesis using an inhibitor of 20-alpha-cholesterol oxidase was without effect on meiotic progression or sperm penetration but reduced male pronuclear formation. The combined results demonstrate the importance of appropriate somatic support of oocytes as measured by their subsequent capacity to induce normal pronuclear formation after fertilization. Furthermore, studies on the required hormonal environment support published data showing that progesterone is required during maturation for subsequent male pronuclear formation.

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Accession: 002355945

PMID: 8501438

DOI: 10.1002/jez.1402660208

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Davidson J.M.; Mceneany L.S.G.; Bornstein P., 1979: Pro collagen processing limited proteolysis of carboxyl terminal extension peptides by a cathepsin like protease secreted by tendon fibroblasts. An enzymatic activity, capable of removing the COOH-terminal extensions of type I chick procollagen was demonstrated in embryonic chick tendons and in cultured tendon fibroblasts utilizing 2 new methods of analysis. The protease was purified by a...

NEUFELD.H.N.; WAGENVOORT.C.A.; ONGLEY.P.A.; EDWARDS.J.E., 1962: Hypoplasia of ascending aorta. An unusual form of supravalvular aortic stenosis with special reference to localized coronary arterial hypertension. American Journal of Cardiology 10: 746-751

Forgeron, M.A.M.; Wasylishen, R.E., 2008: Molybdenum magnetic shielding and quadrupolar tensors for a series of molybdate salts: a solid-state 95Mo NMR study. A series of molybdate, MoO4(2-), salts have been studied using solid-state 95Mo NMR spectroscopy at applied magnetic field strengths of 11.75, 17.63 and 21.14 T. In contrast to previous investigations, the principal components of the Mo shielding...

Simma, B.; Skladal, D.; Falk, M., 2000: Predicting survival infants ventilated with high-frequency oscillation. This retrospective study identifies factors which may predict outcome in preterm infants and infants born at term, ventilated with high-frequency oscillation (HFO). In a 16-bed neonatal and paediatric intensive care unit (level III), 58 consecutiv...

Boonyaratanakornkit, B.; Simpson, A.J.; Park, C.; Fraser, C.; E.S.yed, N.M.A.; Clark, D., 2002: Expression profiles of the hyperthermophilic archaeon Methanococcus jannaschii under extreme temperatures and pressures. We have monitored RNA expression on a genome-wide scale through the use of cDNA microarrays developed for the hyperthermophilic archaeon Methanococcus jannaschii. M. jannaschii was chosen for this study due to its ability to survive under extreme...

Banks, Charles S., 1907: Experiments in malarial transmission by means of Myzomyia ludlowii Theob. Philippine Journal of Science B: 2 513-535

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Plewig, G.; Dressel, H.; Pfleger, M.; Michelsen, S.; Kligman, A.M., 2005: Low dose isotretinoin combined with tretinoin is effective to correct abnormalities of acne. Isotretinoin is well known in the therapy of acne papulopustulosa and acne conglobata. No study has investigated the pathophysiological changes of the skin of acne patients, especially when low dose oral isotretinoin is given in combination with t...

Lesage, L.; Harper, P.P., 1976: Life cycles of elmidae coleoptera inhabiting streams of the laurentian highlands quebec canada. Five stream-dwelling elmid species were studied and each lives in a particular substratum : Stenelmis crenata (Say) on sandy bottoms, Optioservus ampliatus (Fall) within gravel, Macronychus glabratus Say on submerged decaying wood, Promoresia tard...

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