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Effect of gamma irradiation on in vitro androgenesis in rice (Oryza sativa L.)






Oryza 28(3): 305-308

Effect of gamma irradiation on in vitro androgenesis in rice (Oryza sativa L.)

Gamma-irradiated (0.5-3.0 kR) and non-irradiated anthers of rice IR50 were cultured in vitro and examined under the microscope at 3-day intervals. The non-irradiated anthers dehisced at 13-15 days and the microspores formed callus after 26 days. Embryoids formed at 37-40 days and plantlets subsequently regenerated. The irradiated anthers showed degeneration of the tapetal cells 7 days after irradiation. At the higher doses, the microspores failed to progress beyond the uninucleate stage.


Accession: 002356094



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