EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Efficiency of amino acid transport into preserved brush border membrane vesicles from lepidopteran larval midgut


Comparative Biochemistry & Physiology A Comparative Physiology 103(1): 65-71
Efficiency of amino acid transport into preserved brush border membrane vesicles from lepidopteran larval midgut
The effect of different conditions of preservation of the larval midgut tissue of Bombyx mori and Philosamia cynthia on the efficiency of amino acid transport into brush border membrane vesicles (BBMV) has been studied. Freezing and storage of the tissue in liquid nitrogen followed by rapid thawing at 37 degree C allowed the preparation of BBMV which retained fully for B. mori and partially for P. cynthia the ability to perform L-histidine transport and accumulation. BBMV prepared from fresh midguts of B. mori, immediately frozen and preserved in liquid nitrogen for different periods, lost part of their transport efficiency at the moment of freezing, independently of the time of storage. A decrease was observed for preserved BBMV from P. cynthia midgut. However, the addition of a cryoprotectant such as glycerol to BBMV improved the performance of the reconstituted preparation. The transport capacity of the stored vesicles depended also on their slow thawing on ice.

Accession: 002367904

DOI: 10.1016/0300-9629(92)90242-I

Download PDF Full Text: Efficiency of amino acid transport into preserved brush border membrane vesicles from lepidopteran larval midgut



Related references

Amino acid transport systems in brush-border membrane vesicles from lepidopteran enterocytes. Journal of Experimental Biology, 143: 87-100, 1989

L- and D-alanine transport in brush border membrane vesicles from lepidopteran midgut: evidence for two transport systems. Journal of Membrane Biology 81(3): 233-240, 1984

Kinetics of leucine transport in brush border membrane vesicles from lepidopteran larvae midgut. Journal of Biological Chemistry 267(22): 15391-7, 1992

Substrate structure and amino acid/K+ symport in brush-border membrane vesicles from larval Manduca sexta midgut. Journal of Experimental Biology 197: 237-250, 1994

Potential differences influence amino acid/Na+ symport rates in larval Manduca sexta midgut brush-border membrane vesicles. Journal of Experimental Biology 189: 55-67, 1994

Arginine uptake through a novel cationic amino acid:K+ symporter, System R+, in brush border membrane vesicles from larval Manduca sexta midgut. Biochimica et Biophysica Acta 1282(1): 25-31, 1996

Arginine uptake through a novel cationic amino acid:K+ symporter, system R+, in brush border membrane vesicles from larval Manduca sexta midgut. Biochimica et Biophysica Acta 1282(1): 25-31, 1996

Interaction between Na+ and the K+-dependent amino acid transport in midgut brush-border membrane vesicles from Philosamia cynthia larvae. Journal of Insect Physiology 40(1): 69-74, 1994

Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the gypsy moth (Lymantria dispar). Archives of Insect Biochemistry and Physiology 24(3): 139-147, 1993

Preparation and partial characterization of amino acid transporting brush border membrane vesicles from larval midgut of the cabbage butterfly (Pieris brassicae). Comparative Biochemistry and Physiology A: 862: 301-308, 1987