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Estimation and Analysis of Cucumber (Cucumis sativus L.) Leaf Cellular Heat Sensitivity

Plant Physiology 101(3): 939-945
Estimation and Analysis of Cucumber (Cucumis sativus L.) Leaf Cellular Heat Sensitivity
Triphenyl tetrazolium chloride (TTC) reduction by cucumber (Cucumis sativus L. cv Poinsett 76 and cv Ashley) leaf discs was used as a viability assay to examine the effect of temperature pretreatment on the tissue response to acute hyperthermia. Semi-logarithmic plots of TTC reduction as a function of incubation time at different temperatures from 40 to 60 degrees C resembled the heat survival curves of animal cells. Heat inactivation rates were obtained and subjected to "quasi" Arrhenius analyses by analytical methods derived from the animal studies. The Arrhenius plots of TTC reduction rates for cv Ashley leaf discs preincubated at 25 or 37 degrees C and for cv Poinsett 76 preincubated at 37 degrees C were linear with the same activation energy (Ea) of about 80 kcal mol-1. The Arrhenius plot of cv Poinsett 76 preincubated at 25 degrees C was nonlinear with an Ea of about 80 kcal mol-1 at temperatures below 46 degrees C and an Ea of about 27.5 kcal mol-1 at temperatures above 47 degrees C. The significance of these differences is discussed in terms of the role of protein denaturation in the thermal sensitivity of cucumber disc reduction of TTC and the applicability of these methods to the analysis of plant cellular heat sensitivity.

Accession: 002373075

PMID: 12231745

DOI: 10.2307/4275055

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