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Estimation of adenosine-5'-triphosphate by bioluminescence and its use as an indicator of bacterial biomass in rumen liquid


, : Estimation of adenosine-5'-triphosphate by bioluminescence and its use as an indicator of bacterial biomass in rumen liquid. Zootecnica e Nutrizione Animale 17(4): 227-238

Rumen fluid was sampled at 8.30 and 14.30 h during 3 consecutive days in 2 groups of Italian Red Pied cows with fistulae, given diets of grass haylage, sugarbeet pulp molasses and soyabean meal 77:17:6 (diet F) or maize silage, permanent pasture hay and concentrate 25:25:50 (diet C). Rumen bacteria were isolated by centrifugation at 22 500 g and contents of DM, N and adenosine-5'-triphosphate (ATP) estimated. Estimation of ATP was by bioluminescence after extraction with tris-EDTA.

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Related references

Forsberg, C.W.; Lam, K., 1977: Use of adenosine 5'-triphosphate as an indicator of the microbiota biomass in rumen contents. A number of techniques were tested for their efficiency in extracting adenosine 5'-triphosphate (ATP) from strained rumen fluid (SRF). Extraction with 0.6 N H(2)SO(4), using a modification of the procedure described by Lee et al. (1971), was...

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