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Estimation of condensed tannins in faba bean (Vicia faba L.)


, : Estimation of condensed tannins in faba bean (Vicia faba L.). Recent advances of research in antinutritional factors in legume seeds: 117-120

A novel nylon chromatographic method for quantitative estimation of condensed tannins (proanthocyanidins) in the testa was developed and tested on 5 heterogeneous plant introductions of different provenance. Separation according to testa colour yielded 22 samples for analysis, some of which were analysed by the vanillin method for comparison.

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Related references

Van der Poel, A.F.; Dellaert, L.M.; Van Norel, A.; Helsper, J.P., 1992: The digestibility in piglets of faba bean (Vicia faba L.) as affected by breeding towards the absence of condensed tannins. Seed samples from two near-isogenic faba bean (Vicia faba L.) lines were examined for the levels of so-called anti-nutritional factors (ANF). From the ANF known to be present in faba beans, trypsin inhibitor activity, functional lectins, condensed...

Wang P X.; Ueberschaer K.H., 1990: The estimation of vicine convicine and condensed tannins in 22 varieties of faba beans vicia faba l. Using the high-performance reversed-phase liquid chromatography (HPLC) method, 20 varieties of mature fababeans were shown to contain 6.68 .+-. 1.12 mg vicine, 2.33 .+-. 0.59 mg convicine and 9.02 .+-. 1.32 mg (vicine + convicine) g-1 air-dry bean...

Jansman, A.J.M.; Enting, H.; Verstegen, M.W.A.; Huisman, J., 1994: Effect of condensed tannins in hulls of faba bean (Vicia faba L.) on the activities of trypsin (EC 2.4.21.4) and chymotrypsin (EC 2.4.21.1) in digesta collected from the small intestine of pigs. The effects of condensed tannins in hulls of faba beans (Vicia faba L.) on the activities of trypsin (EC 3.4.21.4) and chymotrypsin (EC3.4.21.1) in digesta obtained from the small intestine of pigs were studied. Using four castrated male pigs (mea...

Zakrzewska, E., 1985: Reaction of small faba bean (Vicia faba L. var. minor Harz.) and faba bean (Vicia faba L. var. faba) to Ascochyta fabae Speg. Seed losses of 5-50% are caused by the pathogen and may be significantly higher under conditions favourable for disease development. Breeding resistant cultivars and cultural practices to improve plant health are recommended.

Helsper, J., P.F.G.; Hoogendijk, J., M.; Van-Norel, A.; Burger-Meyer, K., 1993: Antinutritional factors in faba beans (Vicia faba L.) as affected by breeding toward the absence of condensed tannins. The effect of genetic removal of condensed tannins on the levels of other antinutritional factors in faba beans (Vicia faba L.) has been studied by comparison between partners of six near-isogenic pairs for trypsin inhibitor activities (TIA), conc...

Helsper, J.P.F.G.; Norel, A. van; Burger Meyer, K.; Hoogendijk, J.M., 1994: Effect of the absence of condensed tannins in faba beans (Vicia faba) on resistance to foot rot, Ascochyta blight and chocolate spot. The effect of breeding for the absence of condensed tannins in the testa of faba beans on resistance to foot rot, Ascochyta blight and chocolate spot diseases was investigated during 1988-92 at Wageningen, Netherlands. Disease development in tanni...

Helsper, J.; Norel, A.V.n; Burger-Meyer, K.H.ogendijk, J., 1994: Effect of the absence of condensed tannins in faba beans (Vicia faba) on resistance to root rot, Ascochyta blight and chocolate spot. Journal of agricultural science 123(3): 349-355

Olsen, H.S.Jr ; Andersen, J.H., 1978: The estimation of vicine and convicine in faba beans vicia faba and isolated faba bean proteins. A specific method of analysis for each of the pyrimidine glucosides, vicine and convicine, is described. This involves extraction by ethanol, evaporation, separation on TLC and quantitative UV spectrophotometry. Using this method mature dehulled f...

Leeuwen, P. van; Koninkx, J.F.J.G.; Jansman, A.J.M.; Mouwen, J.M.V.M., 1993: Effects of condensed tannins in hulls of faba beans (Vicia faba L.) on some morphological and functional parameters of the small intestinal mucosa of piglets. The apparent digestibility of protein from varieties with relatively high contents of condensed tannins in the seeds is generally lower than in varieties with a low content of tannins, suggesting that the binding of tannins in the diet with protei...

Jansman, A.J.M.; Verstegen, M.W.A.; Huisman, J., 1993: Effects of dietary inclusion of hulls of faba beans (Vicia faba L.) with a low and high content of condensed tannins on digestion and some physiological parameters in piglets. 24 weaned crossbred pigs, 8-17 kg, were fed on diets containing tannin-free, autoclaved (120 degrees C, 20 min) pea hulls 152 g/kg (control) or faba bean hulls of a white-flowering variety (Blandine) with low condensed tannin content (<0.10% ca...